Host-range determinants on the PB2 protein of influenza A viruses control the interaction between the viral polymerase and nucleoprotein in human cells

被引：174

作者：

Labadie, Karine ^{[1
]}

Afonso, Emmanuel Dos Santos ^{[1
]}

Rameix-Welti, Marie-Anne ^{[1
]}

van der Werf, Sylvie ^{[1
]}

Naffakh, Nadia ^{[1
]}

机构：

[1] Univ Paris 07, Unite Genet Mol Virus Resp, URA 1966,Inst Pasteur, CNRS,EA 302, F-75724 Paris 15, France

来源：

VIROLOGY | 2007年 / 362卷 / 02期

关键词：

influenza A virus; host-range; transcription/replication; PB2; NP; RECEPTOR SPECIFICITY; REASSORTANT VIRUSES; REVERSE GENETICS; RNA REPLICATION; MOLECULAR-BASIS; HIGH VIRULENCE; H5N1; VIRUSES; AMINO-ACID; GENES; BINDING;

D O I：

10.1016/j.virol.2006.12.027

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The transcription/replication activity of ribonucleoproteins derived from influenza A primary isolates of human (A/Paris/908/97) or avian origin (A/Mallard/Marquenterre/MZ237/83, A/Hong Kong/156/97) was compared upon reconstitution in mammalian or avian cells, using viral-like reporter RNAs synthesized under the control of the human and chicken RNA polymerase I promoters, respectively. In avian cells, transcription/replication activities were in the same range with all ribonucleoproteins tested. In human cells, ribonucleoproteins derived from A/ Mallard/Marquenterre/MZ237/83 showed reduced transcription/replication activity and reduced NP binding to the PB1-PB2-PA complex (P) or to the isolated PB2 subunit, as compared to the ribonucleoproteins derived from A/Paris/908/97. Both defects were restored when PB2 residue Glu-627 was changed to a Lys. Ribonucleoproteins derived from the human A/Hong Kong/156/97 H5N1 isolate showed efficient NP-P interaction in human cells, and high levels of activity which were determined mostly by the PB2 and PA proteins. Our data suggest that PB2 might play a pivotal role in molecular interactions involving both the viral nucleoprotein and cellular proteins. (C) 2007 Elsevier Inc. All rights reserved.

引用

页码：271 / 282

页数：12

共 46 条

[1] The generation of recombinant influenza A viruses expressing a PB2 fusion protein requires the conservation of a packaging signal overlapping the coding and noncoding regions at the 5′ end of the PB2 segment [J].