Probable laboratory contamination of clinical specimens with Leptospira meyeri

被引：5

作者：

Koizumi, N ^{[1
]}

Kawabata, H ^{[1
]}

Watanabe, H ^{[1
]}

机构：

[1] Natl Inst Infect Dis, Dept Bacteriol, Shinjuku Ku, Tokyo 1628640, Japan

来源：

MICROBIOLOGY AND IMMUNOLOGY | 2003年 / 47卷 / 04期

关键词：

Leptospira meyeri; contamination;

D O I：

10.1111/j.1348-0421.2003.tb03400.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Leptospira meyeri were isolated from the blood or the pleural effusion cultures of four patients at a commercial clinical laboratory. All isolates were identical in their 16S rDNA sequences and NotI or SfiI restriction profiles of the chromosome DNA on pulsed-field gel electrophoresis. However, intraperitoneal inoculation of the isolate (NIID1) failed to kill hamsters and none of the patients' sera reacted with L meyeri strains isolated, indicating leptospiral contamination occurred during laboratory investigation.

引用

页码：305 / 306

页数：2

共 6 条

[1] CONTAMINATION OF BACTERIOLOGICAL MEDIA BY LEPTOSPIRA-BIFLEXA [J].

BRENDLE, JJ ;

ALEXANDE.AD .

APPLIED MICROBIOLOGY, 1974, 28 (03) :505-506

[2]

Faine S., 1999, LEPTOSPIRA LEPTOSPIR

[3] PULSED-FIELD GEL-ELECTROPHORESIS OF NOTI DIGESTS OF LEPTOSPIRAL DNA - A NEW RAPID METHOD OF SEROVAR IDENTIFICATION [J].

HERRMANN, JL ;

BELLENGER, E ;

PEROLAT, P ;

BARANTON, G ;

SAINTGIRONS, I .

JOURNAL OF CLINICAL MICROBIOLOGY, 1992, 30 (07) :1696-1702

[4] Leptospirosis [J].

Levett, PN .

CLINICAL MICROBIOLOGY REVIEWS, 2001, 14 (02) :296-+

[5] Interest of partial 16S rDNA gene sequences to resolve heterogeneities between Leptospira collections:: application to L-meyeri [J].

Postic, D ;

Riquelme-Sertour, N ;

Merien, F ;

Perolat, P ;

Baranton, G .

RESEARCH IN MICROBIOLOGY, 2000, 151 (05) :333-341

[6] ISOLATION OF LEPTOSPIRA-BIFLEXA FROM COMMERCIALLY PREPARED DEIONIZED WATER LABELED STERILE FOR TISSUE-CULTURE [J].

RUBIN, SJ ;

PERLMAN, S ;

ELLINGHAUSEN, HC .

JOURNAL OF CLINICAL MICROBIOLOGY, 1980, 12 (01) :121-123

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