A33scFv-cytosine deaminase: a recombinant protein construct for antibody-directed enzyme-prodrug therapy

被引:36
作者
Deckert, PM [1 ]
Renner, C
Cohen, LS
Jungbluth, A
Ritter, G
Bertino, JR
Old, LJ
Welt, S
机构
[1] Free Univ Berlin, Klinikum Benjamin Franklin, Med Klin 3, D-12200 Berlin, Germany
[2] Univ Saarlandes Kliniken, Med Klin 1, D-66421 Homburg, Germany
[3] Ludwig Inst Canc Res, New York Branch, New York, NY 10021 USA
[4] Mem Sloan Kettering Canc Ctr, New York, NY 10021 USA
关键词
tumour targeting; A33; antibody; antibody directed enzyme-producing therapy (ADEPT); colon carcinoma; recombinant fusion proteins;
D O I
10.1038/sj.bjc.6600751
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
A recombinant fusion protein of colon carcinoma binding A33 single chain antibody with cytosine deaminase displayed specific antigen binding and enzyme activity in surface plasmon resonance and is catalytic activity assay. In vitro, it selectively increased the toxicity of 5-FC to A33 antigen-positive cells by 300-fold, demonstrating the potency of this ADEPT strategy, (C) 2003 Cancer Research UK.
引用
收藏
页码:937 / 939
页数:3
相关论文
共 11 条
  • [1] AUSTIN EA, 1993, MOL PHARMACOL, V43, P380
  • [2] Bhatia J, 2000, INT J CANCER, V85, P571
  • [3] Kinetic analysis of the interaction between the monoclonal antibody A33 and its colonic epithelial antigen by the use of an optical biosensor - A comparison of immobilisation strategies
    Catimel, B
    Nerrie, M
    Lee, FT
    Scott, AM
    Ritter, G
    Welt, S
    Old, LJ
    Burgess, AW
    Nice, EC
    [J]. JOURNAL OF CHROMATOGRAPHY A, 1997, 776 (01) : 15 - 30
  • [4] Deckert PM, 2000, INT J CANCER, V87, P382, DOI 10.1002/1097-0215(20000801)87:3<382::AID-IJC12>3.3.CO
  • [5] 2-G
  • [6] GarinChesa P, 1996, INT J ONCOL, V9, P465
  • [7] The structure of Escherichia coli cytosine deaminase
    Ireton, GC
    McDermott, G
    Black, ME
    Stoddard, BL
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 2002, 315 (04) : 687 - 697
  • [8] The rabbit antibody repertoire as a novel source for the generation of therapeutic human antibodies
    Rader, C
    Ritter, G
    Nathan, S
    Elia, M
    Gout, I
    Jungbluth, AA
    Cohen, LS
    Welt, S
    Old, LJ
    Barbas, CF
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (18) : 13668 - 13676
  • [9] WALLACE PM, 1994, CANCER RES, V54, P2719
  • [10] Measurement of the critical DNA lesions produced by antibody-directed enzyme prodrug therapy (ADEPT) in vitro, in vivo and in clinical material
    Webley, SD
    Francis, RJ
    Pedley, RB
    Sharma, SK
    Begent, RHJ
    Hartley, JA
    Hochhauser, D
    [J]. BRITISH JOURNAL OF CANCER, 2001, 84 (12) : 1671 - 1676