A gridded genomic library of the honeybee (Apis mellifera):: A reference library system for basic and comparative genetic studies of a hymenopteran genome

被引:19
作者
Beye, M
Poch, A
Burgtorf, C
Moritz, RFA
Lehrach, H
机构
[1] Tech Univ Berlin, Inst Okol & Biol, D-10587 Berlin, Germany
[2] Martin Luther Universitat Halle Wittenberg, Inst Zool, D-06099 Halle, Germany
[3] MPI, DHGP, Ressourcezentrum, D-14059 Berlin, Germany
[4] Max Planck Inst Mol Genet, D-14195 Berlin, Germany
关键词
D O I
10.1006/geno.1998.5253
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
We present a gridded genomic library of the honeybee (Apis mellifera) for comparative and basic genetic study of the honeybee genome. The library will be established as a "Reference Library" system, and clones as well as data will be shared with the entire scientific community. This will accelerate the molecular level of honeybee genetics, combining the efforts of different laboratories. Because of male haploidy and the high rate of recombination, the honeybee is becoming a model organism for genomic studies of naturally occurring traits and behavioral genetics. The library consists of about 110,000 clones spotted at high density onto four filter membranes, representing 22 genome equivalents. Preliminary analysis using single-copy sequences revealed a positive clone number of the same order. The techniques for library generation and preliminary analysis as well as library access are described. (C) 1998 Academic Press.
引用
收藏
页码:317 / 320
页数:4
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