Transcriptional analysis of inducible acetamidase gene of Mycobacterium smegmatis

被引:12
作者
Narayanan, S [1 ]
Selvakumar, S [1 ]
Aarati, R [1 ]
Vasan, SK [1 ]
Narayanan, PR [1 ]
机构
[1] TB Res Ctr, Dept Immunol, Chetput 600031, Chennai, India
关键词
acetamidase; gene regulation; transcription start site; promoter; Mycobacterium smegmatis;
D O I
10.1016/S0378-1097(00)00447-X
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Isolation and analysis of strong and regulatable promoters of mycobacteria should be useful tools to aid the expression of cloned genes in mycobacteria. In the present study, we have mapped the transcriptional start site of the inducible acetamidase gene of Mycobacterium smegmatis and studied the mechanism of its regulation. Northern blot, reverse transcription-PCR and primer extension analysis studies were used to determine the position of the promoter. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
引用
收藏
页码:263 / 268
页数:6
相关论文
共 18 条
[1]   Identification and analysis of "extended -10" promoters from mycobacteria [J].
Bashyam, MD ;
Tyagi, AK .
JOURNAL OF BACTERIOLOGY, 1998, 180 (09) :2568-2573
[2]   EVIDENCE FOR THE OCCURRENCE OF PERMEASES FOR TRICARBOXYLIC ACID CYCLE INTERMEDIATES IN PSEUDOMONAS-AERUGINOSA [J].
CLARKE, PH ;
MEADOW, PM .
JOURNAL OF GENERAL MICROBIOLOGY, 1959, 20 (01) :144-155
[3]  
DRAPER P, 1967, J GEN MICROBIOL, V46, P11
[4]   2 PROMOTERS, ONE INDUCIBLE AND ONE CONSTITUTIVE, CONTROL TRANSCRIPTION OF THE STREPTOMYCES-LIVIDANS GALACTOSE OPERON [J].
FORNWALD, JA ;
SCHMIDT, FJ ;
ADAMS, CW ;
ROSENBERG, M ;
BRAWNER, ME .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (08) :2130-2134
[5]  
HALPERN VS, 1957, BIOCHEM J, V65, P716
[6]   BACTERIAL PROTOPLAST FUSION - RECOMBINATION IN FUSED PROTOPLASTS OF STREPTOMYCES-COELICOLOR [J].
HOPWOOD, DA ;
WRIGHT, HM .
MOLECULAR & GENERAL GENETICS, 1978, 162 (03) :307-317
[7]  
Kohn HI, 1941, J PHARMACOL EXP THER, V73, P343
[8]   THE MINUS 35-RECOGNITION REGION OF ESCHERICHIA-COLI SIGMA-70 IS UNESSENTIAL FOR INITIATION OF TRANSCRIPTION AT AN EXTENDED MINUS 10 PROMOTER [J].
KUMAR, A ;
MALLOCH, RA ;
FUJITA, N ;
SMILLIE, DA ;
ISHIHAMA, A ;
HAYWARD, RS .
JOURNAL OF MOLECULAR BIOLOGY, 1993, 232 (02) :406-418
[9]   CLONING AND SEQUENCING OF THE GENE WHICH ENCODES THE HIGHLY INDUCIBLE ACETAMIDASE OF MYCOBACTERIUM-SMEGMATIS [J].
MAHENTHIRALINGAM, E ;
DRAPER, P ;
DAVIS, EO ;
COLSTON, MJ .
JOURNAL OF GENERAL MICROBIOLOGY, 1993, 139 :575-583
[10]   Induction of haemolytic activity in Escherichia coli by the slyA gene product [J].
Oscarsson, J ;
Mizunoe, Y ;
Uhlin, BE ;
Haydon, DJ .
MOLECULAR MICROBIOLOGY, 1996, 20 (01) :191-199