Chemical amplification: Continuous-flow PCR on a chip

被引：1074

作者：

Kopp, MU ^{[1
]}

de Mello, AJ ^{[1
]}

Manz, A ^{[1
]}

机构：

[1] Univ London Imperial Coll Sci Technol & Med, Zeneca SmithKline Beecham Ctr Analyt Sci, Dept Chem, London SW7 2AY, England

来源：

SCIENCE | 1998年 / 280卷 / 5366期

关键词：

D O I：

10.1126/science.280.5366.1046

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A micromachined chemical amplifier was successfully used to perform the polymerase chain reaction (PCR) in continuous flow at high speed. The device is analogous to an electronic amplifier and relies on the movement of sample through thermostated temperature zones on a glass microchip. Input and output of material (DNA) is continuous, and amplification is independent of input concentration. A 20-cycle PCR amplification of a 176-base pair fragment from the DNA gyrase gene of Neisseria gonorrhoeae was performed at various flow rates, resulting in total reaction times of 90 seconds to 18.7 minutes.

引用

页码：1046 / 1048

页数：3

共 11 条

[1] Analysis of ligase chain reaction products amplified in a silicon-glass chip using capillary electrophoresis [J].