Effect of the glass surface modification on the strength of methacrylate monolith attachment

被引：41

作者：

Vidic, J ^{[1
]}

Podgornik, A ^{[1
]}

Strancar, A ^{[1
]}

机构：

[1] BIA Separat DOO, SI-1000 Ljubljana, Slovenia

来源：

JOURNAL OF CHROMATOGRAPHY A | 2005年 / 1065卷 / 01期

关键词：

silanization; covalent attachment; monoliths; capillary columns;

D O I：

10.1016/j.chroma.2004.11.044

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The influence of glass surface modification in order to determine strength of the monolith attachment was studied. Modification consists of pre-treatment of the glass with chemicals or boiling in deionized water, silanization and drying has been investigated on different types of glass. Amount of silane groups was determined by measurement of the contact angle between the glass surface and water drop. The highest values were found for soda-lime glass. Strength of the monolith attachment was established by pumping ethanol through the monolithic capillaries and measuring the pressure drop at which monolith was dislodged. Surprisingly, it was found that the critical part of the glass surface modification procedure is glass pre-treatment. Good results were obtained with glass boiled in water for 2.5 h or more. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：51 / 58

页数：8

共 35 条

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