Individual pineal cells in chick possess photoreceptive, circadian clock and melatonin-synthesizing capacities in vitro

Chick pineal cells express a circadian rhythm of melatonin release under light-dark (LD) cycles, with an increase during the dark period and a decrease during the light period, and this rhythm persists under constant darkness (DD). We cultured individual single pineal cells with 15 mu l of medium per well in a Terasaki plate and measured melatonin secretion every 12 h under LD, DL and DD. Individual cells secreted more melatonin during the dark period than during the light period under both LD and DL conditions, and those rhythmic secretions persisted under DD, These results suggest that individual pineal cells in chick have photoreceptive, circadian clock and melatonin-synthesizing capacities. (C) 1997 Elsevier Science B.V.