Cell interaction studies of PLA-MePEG nanoparticles

被引：40

作者：

Nguyen, CA ^{[1
]}

Allémann, E ^{[1
]}

Schwach, G ^{[1
]}

Doelker, E ^{[1
]}

Gurny, R ^{[1
]}

机构：

[1] Univ Geneva, Sch Pharm, CH-1211 Geneva 4, Switzerland

来源：

INTERNATIONAL JOURNAL OF PHARMACEUTICS | 2003年 / 254卷 / 01期

关键词：

nanoparticles; surface modification; PLA-MePEG; flow cytometry; cell interaction; MPS;

D O I：

10.1016/S0378-5173(02)00685-3

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Poly(D,L-lactic acid)-methoxypoly(ethylene glycol) (PLA-MePEG) copolymers were synthesized by ring-opening polymerization of D,L-lactide in the presence of MePEG of different molecular weights and stannous octoate as the catalyst. The chemical composition of the diblock-copolymer PLA-MePEG was confirmed by H-1-NMR and the molecular weight and distribution were assessed by gel permeation chromatography. Nanoparticles containing Nile red as a fluorescent dye were prepared using poly(D,L-lactic acid) (PLA), blends of PLA and PLA-MePEG or PLA-MePEG alone. Incubation of nanoparticles with human blood monocytes was performed in serum or in PBS and the cell-associated fluorescence was analyzed by flow cytometry. In serum, a protective effect was obtained and the interaction of particles with mononuclear leukocytes decreased to 40%. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：69 / 72

页数：4

共 8 条

[1] PREPARATION OF AQUEOUS POLYMERIC NANODISPERSIONS BY A REVERSIBLE SALTING-OUT PROCESS - INFLUENCE OF PROCESS PARAMETERS ON PARTICLE-SIZE [J].