Identification of necrophagous fly species using ISSR and SCAR markers

被引：15

作者：

He, Lin

Wang, Sibao

Miao, Xuexia

Wu, Hong

Huang, Yongping

机构：

[1] Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Plant Physiol & Ecol, Shanghai 200032, Peoples R China

[2] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

来源：

FORENSIC SCIENCE INTERNATIONAL | 2007年 / 168卷 / 2-3期

关键词：

forensic insects; necrophagous flies; molecular markers; ISSR; SCAR;

D O I：

10.1016/j.forsciint.2006.07.006

中图分类号：

DF [法律]; D9 [法律]; R [医药、卫生];

学科分类号：

0301 ; 10 ;

摘要：

Necrophagous fly is the most common insect evidence collected during a death investigation. We analyzed the DNA polymorphism among the five forensically important fly species, namely, Lucilia sericata, Aldrichina grahami, Chrysomya megacephala, Parasarcophaga crassipalpis and Musca domestica using inter simple sequence repeat (ISSR) method. Nine ISSR primers selected from 18 primers could amplify 105 clear and stable bands, of which 95 bands were polymorphic. Some primers produced completely different band pattern in different species, indicating that they can be used to identify these species. Aiming at obtaining more reliable markers that might be universally used, we started an effort to convert species-specific ISSR fragments into the sequence-characterized amplified region (SCAR) markers that can be used for the molecular diagnosis of the five species. (C) 2006 Elsevier Ireland Ltd. All rights reserved.

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收藏

页码：148 / 153

页数：6

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