Detection and analysis of tracers in experimental drowning

被引:20
作者
Bajanowski, T
Brinkmann, B
Stefanec, AM
Barckhaus, RH
Fechner, G
机构
[1] Univ Munster, Inst Legal Med, D-48149 Munster, Germany
[2] Univ Munster, Inst Med Phys & Biophys, D-48149 Munster, Germany
关键词
experimental drowning; tracer study; electron microscopy; X-ray microanalysis;
D O I
10.1007/s004140050115
中图分类号
DF [法律]; D9 [法律]; R [医药、卫生];
学科分类号
0301 ; 10 ;
摘要
In animal experiments, studies on the mechanisms involved in drowning were carried out using latex and gold tracers of defined size and concentration. The tracers were detectable by fluorescence microscopy (latex tracers) and by electron microscopy (gold tracers) in the lungs, kidneys and lymph nodes and were analysed further by X-ray microanalysis using a transmission scanning electron microscope. Tracers with small diameters were shown to penetrate intercellular gaps of the alveolar epithelium and the larger tracers were incorporated into the epithelial and endothelial cells by active pinocytotic mechanisms thus passing through the air-blood barrier. The detection and analysis of tracers in organs of the systemic circulation originating from the immersion fluid can assist in understanding the pathophysiology of drowning and in some selected cases, in making a more definitive diagnosis.
引用
收藏
页码:57 / 61
页数:5
相关论文
共 30 条
[1]  
BENSCH KG, 1969, SCIENCE, V156, P1204
[2]  
BRINKMANN B, 1983, Z RECHTSMED, V89, P267
[3]  
Chinard F P, 1980, Physiologist, V23, P62
[4]  
CHINARD FP, 1980, MICROVASC RES, V21, P1
[5]  
Fechner G, 1983, Beitr Gerichtl Med, V41, P303
[6]   DIATOMS AND DROWNING - ONCE MORE [J].
FOGED, N .
FORENSIC SCIENCE INTERNATIONAL, 1983, 21 (02) :153-159
[7]   CONTROLLED NUCLEATION FOR REGULATION OF PARTICLE-SIZE IN MONODISPERSE GOLD SUSPENSIONS [J].
FRENS, G .
NATURE-PHYSICAL SCIENCE, 1973, 241 (105) :20-22
[8]  
Gettler A, 1921, J AMER MED ASSOC, V77, P1650
[9]   Interleukin-1 alpha (IL-1 alpha) and N-formyl-methionyl-leucyl-phenylalanine (FMLP) as potential inducers of supravital chemotaxis [J].
Grellner, W ;
Madea, B ;
Kruppenbacher, JP ;
Dimmeler, S .
INTERNATIONAL JOURNAL OF LEGAL MEDICINE, 1996, 109 (03) :130-133
[10]  
HAGEMANN E, 1960, RATTE MAUS VERSUCHST