Antibody-mediated purification of co-expressed antigen-antibody complexes

被引：11

作者：

Bossi, Sergio ^{[1
]}

Ferranti, Benedetta ^{[1
]}

Martinelli, Chiara ^{[2
]}

Capasso, Paola ^{[1
]}

de Marco, Ario ^{[1
]}

机构：

[1] Cogentech IFOM IEO Campus Oncogenom, Prot Chem Unit, I-20139 Milan, Italy

[2] SEMM IFOM IEO Campus Oncogenom, I-20139 Milan, Italy

来源：

PROTEIN EXPRESSION AND PURIFICATION | 2010年 / 72卷 / 01期

关键词：

Single-domain antibodies; Immunoaffinity purification; Protein co-expression; Protein complexes; Llama naive antibody phage display library; VHH format; ESCHERICHIA-COLI; CRYSTAL-STRUCTURE; PROTEINS; CHAPERONE; IMMUNOPURIFICATION; EXPRESSION; FRAGMENTS;

D O I：

10.1016/j.pep.2010.01.003

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

Immunoaffinity is an established chromatographic method for isolating macromolecules independently on the presence of specific tags while the tight interaction between antigen and antibody has been exploited to stabilize proteins during crystallization trials. Therefore, it seems reasonable to try to combine the two protocols, namely to co-express the target proteins together with their specific antibodies to obtain stable complexes suitable for direct purification and further analyses. Using the variable region of single domain llama antibodies, we showed that the co-expression of antigen-antibody pairs is feasible in both the periplasm and the cytoplasm of bacteria. Moreover, the complexes that were formed in vivo could be purified using a tag fused to the recombinant antibody and remained stable during gel-filtration. The co-expression and co-purification strategy significantly increased the final protein yields promoting the accumulation of functional intrabodies. The described method may offer a suitable alternative for the purification of proteins intended for crystallization trials and it may also be used as a general purification protocol for both antigens and recombinant antibodies. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：55 / 58

页数：4

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