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Folic acid-conjugated protein cages of a plant virus: A novel delivery platform for doxorubicin

被引：172
作者：
Ren, Yupeng
Wong, Sek Man
Lim, Lee-Yong
机构：
[1] Natl Univ Singapore, Dept Pharm, Singapore 117543, Singapore
[2] Natl Univ Singapore, Dept Biol Sci, Singapore 117543, Singapore
[3] Temasek Life Sci Lab, Singapore 117604, Singapore
[4] Univ Western Australia, Sch Biomed Biomol & Chem Sci, Crawley, WA 6009, Australia
来源：
BIOCONJUGATE CHEMISTRY | 2007年 / 18卷 / 03期
关键词：
D O I：
10.1021/bc060361p
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The protein cage of a plant virus may provide a template for monodispersed nanosized systems for drug delivery. Using the Hibiscus chlorotic ringspot virus (HCRSV) as a model plant virus, we have prepared nanosized protein cages (30 nm) capable of encapsulating the anticancer drug, doxorubicin. The technique utilized the simultaneous encapsulation of a polyprotic acid of mw 200 kDa to produce an encapsulation efficiency for doxorubicin of about 7.5%. Folic acid was conjugated onto the capsids to impart cancer-targeting capability. The resultant nanosized systems improved the uptake and cytotoxicity of doxorubicin in the ovarian cancer cells, OVCAR-3, with statistical significance. Plant virus capsids may therefore provide viable templates for targeted drug delivery in cancer chemotherapy.
引用
收藏
页码：836 / 843
页数：8
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