Molecular cloning of a DEG/ENaC sodium channel cDNA from human testis

被引：40

作者：

Ishibashi, K

Marumo, F

机构：

[1] Tokyo Med & Dent Univ, Dept Internal Med 2, Bunkyo Ku, Tokyo 1138519, Japan

[2] Oume Gen Hosp, Renal Unit, Tokyo 1980042, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1998年 / 245卷 / 02期

关键词：

sodium channel; testis; DRASIC;

D O I：

10.1006/bbrc.1998.8483

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The number of members of the recently defined DEG/ENaC sodium channel superfamily is increasing. Their importance in Na transport, taste perception, acid sensing, and mechanotransduction has been implicated. We have cloned a new member of this superfamily from human testis, which was named hTNaC1 (for human testis sodium channel 1). The hTNaC1 has 532 amino acid residues with two hydrophobic transmembrane domains. It has the highest identity (82%) with a rat H+-gated Na channel specific for sensory neurons (DRASIC) and a low identity (29%) with an epithelial isoform (alpha-ENaC) of this superfamily. Northern blot of human tissues revealed its selective expression in testis (7 kb) and absence in other tissues. The identification of a new member of Na channels specifically expressed in testis will expand the role of this channel family to the reproduction physiology. (C) 1998 Academic Press.

引用

收藏

页码：589 / 593

页数：5

相关论文

共 20 条

[1] The acid-sensitive ionic channel subunit ASIC and the mammalian degenerin MDEG form a heteromultimeric H+-gated Na+ channel with novel properties [J].

Bassilana, F ;

Champigny, G ;

Waldmann, R ;

deWeille, JR ;

Heurteaux, C ;

Lazdunski, M .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (46) :28819-28822

[2] AMILORIDE-SENSITIVE EPITHELIAL NA+ CHANNEL IS MADE OF 3 HOMOLOGOUS SUBUNITS [J].

CANESSA, CM ;

SCHILD, L ;

BUELL, G ;

THORENS, B ;

GAUTSCHI, I ;

HORISBERGER, JD ;

ROSSIER, BC .

NATURE, 1994, 367 (6462) :463-467

[3] Cation and anion channels in fat and human spermatozoa [J].

Chan, HC ;

Zhou, TS ;

Fu, WO ;

Wang, WP ;

Shi, YL ;

Wong, PYD .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 1997, 1323 (01) :117-129

[4] BNaC1 and BNaC2 constitute at new family of human neuronal sodium channels related to degenerins and epithelial sodium channels [J].

GarciaAnoveros, J ;

Derfler, B ;

NevilleGolden, J ;

Hyman, BT ;

Corey, DP .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (04) :1459-1464

[5]

HYNE RV, 1984, J REPROD FERTIL, V70, P83, DOI 10.1530/jrf.0.0700083

[6] Sequence and transmembrane topology of MEC-4, an ion channel subunit required for mechanotransduction in Caenorhabditis elegans [J].

Lai, CC ;

Hong, K ;

Kinnell, M ;

Chalfie, M ;

Driscoll, M .

JOURNAL OF CELL BIOLOGY, 1996, 133 (05) :1071-1081

[7] The IMAGE consortium: An integrated molecular analysis of genomes and their expression [J].

Lennon, G ;

Auffray, C ;

Polymeropoulos, M ;

Soares, MB .

GENOMICS, 1996, 33 (01) :151-152

[8] EXPRESSION AND LOCALIZATION OF AMILORIDE-SENSITIVE SODIUM-CHANNEL INDICATE A ROLE FOR NON-TASTE CELLS IN TASTE PERCEPTION [J].

LI, XJ ;

BLACKSHAW, S ;

SNYDER, SH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (05) :1814-1818

[9] A modulatory subunit of acid sensing ion channels in brain and dorsal root ganglion cells [J].

Lingueglia, E ;

deWeille, JR ;

Bassilana, F ;

Heurteaux, C ;

Sakai, H ;

Waldmann, R ;

Lazdunski, M .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (47) :29778-29783

[10] CLONING OF THE AMILORIDE-SENSITIVE FMRFAMIDE PEPTIDE-GATED SODIUM-CHANNEL [J].

LINGUEGLIA, E ;

CHAMPIGNY, G ;

LAZDUNSKI, M ;

BARBRY, P .

NATURE, 1995, 378 (6558) :730-733