Purification and characterization of a protease from Pseudomonas aeruginosa grown in cutting oil

被引：26

作者：

Karadzic, I

Masui, A

Fujiwara, N

机构：

[1] Technol Res Inst Osaka Prefecture, Izumi, Osaka 5941157, Japan

[2] Sch Med, Dept Chem, YU-11000 Belgrade, Serbia

来源：

JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 2004年 / 98卷 / 03期

基金：

日本学术振兴会;

关键词：

Pseudomonas aeruginosa; protease; cutting oil;

D O I：

10.1016/S1389-1723(04)00258-0

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 0836 [生物工程]; 090102 [作物遗传育种]; 100705 [微生物与生化药学];

摘要：

The Pseudomonas aeruginosa san-ai strain was isolated from water-soluble cutting oil used for cooling and lubrication during industrial metal-working processes. This strain, which is grown in a high alkaline (pH 10) mixture of surfactants and mineral oil, produces an extracellular proteolytic enzyme. We have purified and characterized this 18 kDa protease. The P aeruginosa san-ai protease functions optimally at pH 9.0 and 60degreesC. Additionally, it is a Zn-containing metalloenzyme, and its monomeric structure contains at least one disulfide bond. Because the enzyme is stable in the presence of organic solvents, it is suitable for peptide synthesis. Furthermore, the P aeruginosa san-ai protease could be used in an intelligent drug delivery system (DDS) designed for applications in the metal industry for prevention of putrefaction of cutting oil.

引用

页码：145 / 152

页数：8

共 30 条

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GEL-FILTRATION BEHAVIOUR OF PROTEINS RELATED TO THEIR MOLECULAR WEIGHTS OVER A WIDE RANGE [J].