Detection of Legionella DNA by PCR of whole-blood samples in a mouse model

被引:9
作者
Aoki, S
Hirakata, Y [1 ]
Miyazaki, Y
Izumikawa, K
Yanagihara, K
Tomono, K
Yamada, Y
Tashiro, T
Kohno, S
Kamihira, S
机构
[1] Nagasaki Univ, Sch Med, Dept Lab Med, Nagasaki 8528501, Japan
[2] Nagasaki Univ, Sch Med, Dept Internal Med 2, Nagasaki 8528501, Japan
关键词
D O I
10.1099/jmm.0.04999-0
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A detection system for Legionella DNA in blood samples based on the PCR was developed and evaluated in A/J mice with experimentally induced Legionella pneumonia. Primers were designed to amplify a 106 bp DNA fragment of the 16S rRNA gene specific to Legionella species. The PCR system could detect clinically relevant Legionella species including Legionella pneumophila, Legionella micdadei, Legionella bozemanae, Legionella dumoffii, Legionella longbeachae, Legionella gormanii and Legionella jordanis. The sensitivity of the PCR system was 20 fg extracted DNA. In the mouse model, the blood PCR was compared with results obtained by PCR on bronchoalveolar lavage fluid (BALF) samples, cultures of blood and BALF and detection of Legionella urinary antigen. Blood PCR was positive until 8 days after infection, while BALF PCR became negative on day 4. These results indicate that PCR using blood samples may be a useful, convenient and non-invasive method for the diagnosis of Legionella pneumonia.
引用
收藏
页码:325 / 329
页数:5
相关论文
共 33 条
[1]   Diagnosis by polymerase chain reaction of pneumonia caused by Legionella pneumophila in an immunocompetent child [J].
Aebischer, CC ;
Matter, L ;
Gaia, V ;
Aebi, C .
INFECTION, 1999, 27 (4-5) :280-282
[2]   Evaluation of the Binax and Biotest urinary antigen kits for detection of Legionnaires' disease due to multiple serogroups and species of Legionella [J].
Benson, RF ;
Tang, PW ;
Fields, BS .
JOURNAL OF CLINICAL MICROBIOLOGY, 2000, 38 (07) :2763-2765
[3]  
Breiman R F, 1998, Semin Respir Infect, V13, P84
[4]  
BRIELAND J, 1994, AM J PATHOL, V145, P1537
[5]   CHARACTERIZATION OF GLYCOPEPTIDE-RESISTANT ENTEROCOCCI FROM UNITED-STATES HOSPITALS [J].
CLARK, NC ;
COOKSEY, RC ;
HILL, BC ;
SWENSON, JM ;
TENOVER, FC .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1993, 37 (11) :2311-2317
[6]   Detection of Legionella species in respiratory specimens using PCR with sequencing confirmation [J].
Cloud, JL ;
Carroll, KC ;
Pixton, P ;
Erali, M ;
Hillyard, DR .
JOURNAL OF CLINICAL MICROBIOLOGY, 2000, 38 (05) :1709-1712
[7]  
Domínguez JA, 1998, J CLIN MICROBIOL, V36, P2718
[8]   Etiology of severe pneumonia in the very elderly [J].
El-Solh, AA ;
Sikka, P ;
Ramadan, F ;
Davies, J .
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 2001, 163 (03) :645-651
[9]   DNA-SEQUENCE OF MIP, A LEGIONELLA-PNEUMOPHILA GENE ASSOCIATED WITH MACROPHAGE INFECTIVITY [J].
ENGLEBERG, NC ;
CARTER, C ;
WEBER, DR ;
CIANCIOTTO, NP ;
EISENSTEIN, BI .
INFECTION AND IMMUNITY, 1989, 57 (04) :1263-1270
[10]  
HARRISON TG, 1988, LANCET, V2, P794