Elevated carbon dioxide reduces emission of herbivore-induced volatiles in Zea mays

Terpene volatiles produced by sweet corn (Zea mays) upon infestation with pests such as beet armyworm (Spodoptera exigua) function as part of an indirect defence mechanism by attracting parasitoid wasps; yet little is known about the impact of climate change on this form of plant defence. To investigate how a central component of climate change affects indirect defence, we measured herbivore-induced volatile emissions in plants grown under elevated carbon dioxide (CO2). We found that S. exigua infested or elicitor-treated Z. mays grown at elevated CO2 had decreased emission of its major sesquiterpene, (E)-beta-caryophyllene and two homoterpenes, (3E)-4,8-dimethyl-1,3,7-nonatriene and (3E,7E)-4,8,12-trimethyl- 1,3,7,11-tridecatetraene. In contrast, inside the leaves, elicitor-induced (E)-beta-caryophyllene hyperaccumulated at elevated CO2, while levels of homoterpenes were unaffected. Furthermore, gene expression analysis revealed that the induction of terpene synthase genes following treatment was lower in plants grown at elevated CO2. Our data indicate that elevated CO2 leads both to a repression of volatile synthesis at the transcriptional level and to limitation of volatile release through effects of CO2 on stomatal conductance. These findings suggest that elevated CO2 may alter the ability of Z. mays to utilize volatile terpenes to mediate indirect defenses.