Recombinant cholera toxin B subunit acts as an adjuvant for the mucosal and systemic responses of mice to mucosally co-administered bovine serum albumin

被引：77

作者：

Tochikubo, K ^{[1
]}

Isaka, M ^{[1
]}

Yasuda, Y ^{[1
]}

Kozuka, S ^{[1
]}

Matano, K ^{[1
]}

Miura, Y ^{[1
]}

Taniguchi, T ^{[1
]}

机构：

[1] Nagoya City Univ, Dept Microbiol, Sch Med, Mizuho Ku, Nagoya, Aichi 467, Japan

来源：

VACCINE | 1998年 / 16卷 / 2-3期

关键词：

recombinant cholera toxin B subunit; mucosal adjuvant; bovine serum albumin;

D O I：

10.1016/S0264-410X(97)00194-1

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We examined the mucosal adjuvant activity of recombinant cholera toxin B subunit (rCTB) produced by Bacillus brevis carrying pNU212-CTB by intranasal or oral co-administration of bovine serum albumin (BSA), Intranasal administration stimulated a high level of BSA-specific serum IgG antibody response and BSA-specific IgA antibody responses in the nasal and pulmonary lavages. Oral administration induced a moderate level of BSA-specific serum Ige antibody and a low level of BSA-specific IgA antibody in the large intestinal washes, These results show that CTB alone can act as an intranasal or-oral delivery carrier; it also has strong adjuvant proper-ties for stimulating serum Ige and mucosal IgA immune responses to unrelated non-coupled antigens after intranasal or oral co-immunization. (C) 1997 Elsevier Science Ltd, All rights reserved.

引用

页码：150 / 155

页数：6

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