Application of nitrite reductase from Alcaligenes faecalis S-6 for nitrite measurement

被引：38

作者：

Sasaki, S

Karube, I

Hirota, N

Arikawa, Y

Nishiyama, M

Kukimoto, M

Horinouchi, S

Beppu, T

机构：

[1] Univ Tokyo, Adv Sci & Technol Res Ctr, Meguro Ku, Tokyo 153, Japan

[2] Japan Womens Univ, Dept Chem & Biol Sci, Fac Sci, Bunkyo Ku, Tokyo 112, Japan

[3] Univ Tokyo, Biotechnol Res Ctr, Bunkyo Ku, Tokyo 113, Japan

[4] Univ Tokyo, Dept Biotechnol, Bunkyo Ku, Tokyo 113, Japan

[5] Nihon Univ, Fac Agr & Vet Med, Dept Appl Biol Sci, Kanagawa 252, Japan

来源：

BIOSENSORS & BIOELECTRONICS | 1998年 / 13卷 / 01期

关键词：

nitrite sensor; nitrite; nitrite reductase; acid rain;

D O I：

10.1016/S0956-5663(97)00100-0

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

The enzymatic reaction of nitrite reductase (NIR) from Alcaligenes faecalis S-6 was applied to the measurement of nitrite. NIR was immobilized on the surface of a gold electrode using filter paper and a dialysis membrane, and used as a working electrode in a three-electrode system. Amperometric methods were applied using NIR and the electron mediator l-methoxy PMS (1-methoxy-5-methylphenazinium methylsulfate). The decrease in cathodic current showed a correlation to nitrite concentration over the range 0-1 mg/l. Measurements using a batch-flow type system gave a lower detection limit of 0.01 mg/l. This is sufficient for the detection of nitrite in natural waters. (C) Published by Elsevier Science Limited.

引用

页码：1 / 5

页数：5

共 13 条

[1] NANOGRAM NITRITE AND NITRATE DETERMINATION IN ENVIRONMENTAL AND BIOLOGICAL-MATERIALS BY VANADIUM(III) REDUCTION WITH CHEMI-LUMINESCENCE DETECTION [J].