HR22C16: A potent small-molecule probe for the dynamics of cell division

被引：134

作者：

Hotha, S

Yarrow, JC

Yang, JG

Garrett, S

Renduchintala, KV

Mayer, TU

Kapoor, TM

机构：

[1] Rockefeller Univ, Lab Chem & Cell Biol, New York, NY 10021 USA

[2] Harvard Univ, Sch Med, Dept Cell Biol, Boston, MA 02115 USA

[3] Max Planck Inst Biochem, D-82152 Martinsried, Germany

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2003年 / 42卷 / 21期

关键词：

antimitotics; inhibitors; molecular motors; photolysis; solid-phase synthesis;

D O I：

10.1002/anie.200351173

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A high-throughput, microscopy-based chemical-genetic screen identified HR22C16, which causes a monoastral mitotic block, as a small-molecule probe for cell division (see picture). By using a diastereoselective, traceless solid-phase synthesis and biological assays, a more potent HR22C16 analogue was then identified. A photocaging strategy for HR22C16 was also developed to allow fast temporal control over the function of the target protein Eg5.

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页码：2379 / 2382

页数：4

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