Structural study of the O-linked sugar chains of human leukocyte tyrosine phosphatase CD45

被引：31

作者：

Furukawa, K ^{[1
]}

Funakoshi, Y

Autero, M

Horejsi, V

Kobata, A

Gahmberg, CG

机构：

[1] Tokyo Metropolitan Inst Gerontol, Dept Biosignal Res, Itabashi Ku, Tokyo 173, Japan

[2] Univ Tokyo, Inst Med Sci, Dept Biochem, Tokyo 108, Japan

[3] Univ Helsinki, Dept Biosci, Div Biochem, Helsinki, Finland

[4] Acad Sci Czech Republ, Inst Mol Genet, Prague, Czech Republic

[5] Tokyo Metropolitan Inst Gerontol, Directors Off, Tokyo, Japan

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1998年 / 251卷 / 1-2期

关键词：

human leukocyte; CD45; O-linked sugar chain;

D O I：

10.1046/j.1432-1327.1998.2510288.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The O-linked sugar chains of the human leukocyte cell surface glycoprotein CD45 were released as tritium-labeled oligosaccharides by beta-elimination in the presence of (NaBH4)-H-3. Mono Q column chromatography revealed that they comprise neutral (64%) and acidic (36%) oligosaccharides, the latter of which were converted to neutral ones by Arthrobacter ureafaciens sialidase treatment. Structural studies of each oligosaccharide fractionated on a Bio-Gel P-4 column by sequential exoglycosidase digestion and by methylation analysis revealed that human leukocyte CD45 contains mainly core 1 and core 2 oligosaccharides, 15% of which are modified with poly (N-acetyllactosamine) chains in different extensions. CD45 consists of several isoforms which were isolated after cell surface sialic acid residues were labeled by periodate/(NaBH4)-H-3 treatment. Bio-Gel P-6 column chromatography of a mixture of the tritium-labeled glycopeptide/oligosaccharides obtained by pronase-digestion followed by mild alkaline borohydride treatment showed that distribution of the sialylated core 2 oligosaccharides is different among CD45 isoforms.

引用

页码：288 / 294

页数：7

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