Protection against renal disease in (NZB x NZW)F1 lupus-prone mice after somatic B cell gene vaccination with anti-DNA immunoglobulin consensus peptide

被引:20
作者
Ferrera, Francesca
Hahn, Bevra H.
Rizzi, Marta
Anderson, Marissa
FitzGerald, John
Millo, Enrico
Indiveri, Francesco
Shi, Fu-Dong
Filaci, Gilberto
La Cava, Antonio
机构
[1] Univ Calif Los Angeles, Lupus Res Lab, Div Rheumatol, David Geffen Sch Med, Los Angeles, CA 90095 USA
[2] Univ Genoa, Genoa, Italy
[3] St Josephs Hosp, Barrow Neurol Inst, Phoenix, AZ USA
来源
ARTHRITIS AND RHEUMATISM | 2007年 / 56卷 / 06期
关键词
D O I
10.1002/art.22700
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Objective. Ig molecules contain epitopes that can induce T cell-mediated immune responses. B cells can process and present such epitopes and activate T cells. The purpose of the present study was to test our hypothesis that T cells that recognize an Ig consensus sequence presented by B cells will modulate lupus-like disease in mice. Methods. (NZB x NZW)F-1 (NZB/NZW) lupus mice received somatic B cell gene transfer of a DNA plasmid encoding a consensus sequence of T cell determinants of murine anti-DNA IgG or control plasmids. Treated animals were monitored for the production of antibody, the development of renal disease, and the phenotype, number, and function of T cells. Results. Treatment of mice with Ig consensus plasmid induced transforming growth factor beta-producing CD8+,CD28- T cells that suppressed the antigen-specific stimulation of CD4+ T cells in a cell-contact-independent manner, reduced antibody production, retarded the development of nephritis, and improved survival. Significantly, adoptive transfer of CD8+,CD28- T cells from protected mice into hypergammaglobulinemic NZB/NZW mice effectively protected the transferred mice from the development of renal disease. Conclusion. Gene expression of anti-DNA Ig consensus sequence induces immunoregulatory T cells that delay the development of lupus nephritis by suppressing hypergammaglobulinemia and renal disease.
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页码:1945 / 1953
页数:9
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