Efficient mutagenesis of zebrafish by a DNA cross-linking agent

被引:16
作者
Ando, H [1 ]
Mishina, M [1 ]
机构
[1] Univ Tokyo, Sch Med, Dept Mol Neurobiol & Pharmacol, Bunkyo Ku, Tokyo 1130033, Japan
关键词
zebrafish; mutagenesis; deletion; trimethylpsoralen; tectum; sensory neuron;
D O I
10.1016/S0304-3940(98)00132-3
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
We developed a novel procedure for efficient mutagenesis of zebrafish using a DNA cross-linking agent 4,5',8-trimethylpsoralen (TMP), which is known to frequently induce small deletions in Escherichia coli and Caenorhabditis elegans. A specific-locus test and pilot screenings indicated that the TMP mutagenesis procedure was efficient. To confirm the successful mutagenesis by TMP, we characterized mutants with selective impairments in the nervous system. The no tectal neuron mutation hindered the development of the tectal neurons, while the edawakare mutation resulted in the enhancement of the extension and branching of the peripheral axons of trigeminal ganglion and Rohon-Beard sensory neurons. These results suggest that the TMP mutagenesis will provide an efficient method to isolate and characterize zebrafish mutants at molecular level. (C) 1998 Published by Elsevier Science Ireland Ltd.
引用
收藏
页码:81 / 84
页数:4
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