HPTLC method for quantification of apigenin in the dried root powder of Gmelina arborea Linn.

被引：3

作者：

Dighe, Vidya V. ^{[1
]}

Pathak, Gayatri M. ^{[1
]}

Tulpule, Ketan M. ^{[1
]}

Gokarn, Vijay N. ^{[1
]}

机构：

[1] SP Mandalis Ramnarain Ruia Coll, Dept Chem, Bombay 400019, Maharashtra, India

来源：

JPC-JOURNAL OF PLANAR CHROMATOGRAPHY-MODERN TLC | 2007年 / 20卷 / 03期

关键词：

HPTLC; apigenin; Gmelina arborea Linn; densitometry;

D O I：

10.1556/JPC.20.2007.3.3

中图分类号：

O65 [分析化学];

学科分类号：

070302 [分析化学]; 081704 [应用化学];

摘要：

A sensitive high-performance thin-layer chromatographic method has been established for quantification of apigenin in dried root powder from Gmelina arborea Linn. Chromatographic separation was performed on silica gel plates with chloroform-acetone-formic acid, 7.6:1.6:0.8 (v/v), as mobile phase. The plates were scanned densitometrically at 340 nm. The method was validated for precision and accuracy. The relative standard deviation for instrumental precision, intra-assay precision, and intermediate precision was <2%. Percentage recovery was 98.87.

引用

页码：179 / 182

页数：4

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