Identification and distribution of different mRNA variants produced by differential splicing in the human phosphodiesterase 9A gene

被引：52

作者：

Rentero, C ^{[1
]}

Monfort, A ^{[1
]}

Puigdomènech, P ^{[1
]}

机构：

[1] CSIC, Inst Biol Mol Barcelona, Dept Mol Genet, ES-08034 Barcelona, Spain

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2003年 / 301卷 / 03期

关键词：

cGMP phosphodiesterase; PDE9A; alternative splicing;

D O I：

10.1016/S0006-291X(03)00021-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The transcript population of the human gene coding for a cGMP-dependent phosphodiesterase (PDE9A) has a complex structure. There is a high level of mRNA in intestinal and prostate tissues, a low level in blood, and intermediate in other tissues. More than 20 different variants produced by differential splicing have been observed and new exons have been identified both by PCR amplification and by the analysis of available EST sequences. In all cases the transcriptional start site is the same and no differential splicing is found in the exons coding for the catalytic domain of the protein. In some cases the protein produced by splice variants is truncated. The distribution of the splice variants is not homogeneous among the different tissues studied. The human, but not the mouse, PDE9A gene appears to have a complex regulation of expression by different isoforms. (C) 2003 Elsevier Science (USA). All rights reserved.

引用

页码：686 / 692

页数：7

共 31 条

[1] Expression of cGMP-specific phosphodiesterase 9A mRNA in the rat brain [J].

Andreeva, SG ;

Dikkes, P ;

Epstein, PM ;

Rosenberg, PA .

JOURNAL OF NEUROSCIENCE, 2001, 21 (22) :9068-9076

[2] SEX IN FLIES - THE SPLICE OF LIFE [J].

BAKER, BS .

NATURE, 1989, 340 (6234) :521-524

[3] PRIMARY SEQUENCE OF CYCLIC-NUCLEOTIDE PHOSPHODIESTERASE ISOZYMES AND THE DESIGN OF SELECTIVE INHIBITORS [J].

BEAVO, JA ;

REIFSNYDER, DH .

TRENDS IN PHARMACOLOGICAL SCIENCES, 1990, 11 (04) :150-155

[4] CYCLIC-NUCLEOTIDE PHOSPHODIESTERASES - FUNCTIONAL IMPLICATIONS OF MULTIPLE ISOFORMS [J].

BEAVO, JA .

PHYSIOLOGICAL REVIEWS, 1995, 75 (04) :725-748

[5] Structure, localization, and regulation of cGMP-inhibited phosphodiesterase (PDE3) [J].

Degerman, E ;

Belfrage, P ;

Manganiello, VC .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (11) :6823-6826

[6] Challenge of human Jurkat T-cells with the adenylate cyclase activator forskolin elicits major changes in cAMP phosphodiesterase (PDE) expression by up-regulating PDE3 and inducing PDE4D1 and PDE4D2 splice variants as well as down-regulating a novel PDE4A splice variant [J].

Erdogan, S ;

Houslay, MD .

BIOCHEMICAL JOURNAL, 1997, 321 :165-175

[7] Isolation and differential tissue distribution of two human cDNAs encoding PDE1 splice variants [J].

Fidock, M ;

Miller, M ;

Lanfear, J .

CELLULAR SIGNALLING, 2002, 14 (01) :53-60

[8] Isolation and characterization of PDE9A, a novel human cGMP-specific phosphodiesterase [J].

Fisher, DA ;

Smith, JF ;

Pillar, JS ;

St Denis, SH ;

Cheng, JB .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (25) :15559-15564

[9]

Francis SH, 2001, PROG NUCLEIC ACID RE, V65, P1

[10]

Gitton Y, 2002, NATURE, V420, P586, DOI 10.1038/nature01178

← 1 2 3 4 →