Elemental and redox analysis of single bacterial cells by X-ray microbeam analysis

被引：136

作者：

Kemner, KM

Kelly, SD

Lai, B

Maser, J

O'Loughlin, EJ

Sholto-Douglas, D

Cai, ZH

Schneegurt, MA

Kulpa, CF

Nealson, KH

机构：

[1] Argonne Natl Lab, Div Environm Res, Argonne, IL 60439 USA

[2] Argonne Natl Lab, Adv Photon Source, Argonne, IL 60439 USA

[3] Wichita State Univ, Dept Biol Sci, Wichita, KS 67260 USA

[4] Univ Notre Dame, Dept Biol Sci, Notre Dame, IN 46556 USA

[5] Univ So Calif, Dept Earth Sci, Los Angeles, CA 90089 USA

来源：

SCIENCE | 2004年 / 306卷 / 5696期

关键词：

D O I：

10.1126/science.1103524

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

High-energy x-ray fluorescence measurements were used to make elemental maps and qualitative chemical analyses of individual Pseudomonas fluorescens strain NCIMB 11764 cells. Marked differences between planktonic and adhered cells were seen in the morphology, elemental composition, and sensitivity to Cr(VI) of hydrated cells at spatial scales of 150 nm. This technology can be applied to natural geomicrobiological systems.

引用

页码：686 / 687

页数：2

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