Kinetic study of phenol hydroxylase and catechol 1,2-dioxygenase biosynthesis by Candida tropicalis cells grown on different phenolic substrates

被引：22

作者：

Ahuatzi-Chacón, D ^{[1
]}

Ordorica-Morales, G ^{[1
]}

Ruiz-Ordaz, N ^{[1
]}

Cristiani-Urbina, E ^{[1
]}

Juárez-Ramírez, C ^{[1
]}

Galíndez-Mayer, J ^{[1
]}

机构：

[1] IPN, Escuela Nacl Ciencias Biol, Dept Ingn Bioquim Carpio & Plan Ayala, Ctr Operat Naranjo, Mexico City 06401, DF, Mexico

来源：

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 2004年 / 20卷 / 07期

关键词：

Candida tropicalis; catechol 1,2-dioxygenase; chloroaromatics; co-metabolism; phenol hydroxylase; phenolic compounds;

D O I：

10.1007/s11274-004-2622-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 0836 [生物工程]; 090102 [作物遗传育种]; 100705 [微生物与生化药学];

摘要：

When Candida tropicalis was grown on phenol, catechol or resorcinol, the highest levels of specific activity of phenol hydroxylase (EC. 1.14.13.7) and catechol 1,2-dioxygenase (EC. 1.13.11.1) were attained with phenol. With the three aromatic compounds tested, the yeast cells exhibited sharp peaks of specific activity of both enzymes at particular incubation times. Phenol-induced cells containing high levels of both enzymes were capable of degrading rapidly and without delay 4-chlorophenol and 2,6-dichlorophenol, and to a lesser extend pentachlorophenol. However, the yeast could not grow on chlorophenols as major carbon and energy source.

引用

页码：695 / 702

页数：8

共 52 条

[1]

DEGRADATION OF PENTACHLOROPHENOL BY FIXED FILMS OF WHITE-ROT FUNGI IN ROTATING TUBE BIOREACTORS [J].