Genetic Basis of Tetracycline Resistance in Bifidobacterium animalis subsp lactis

被引：53

作者：

Gueimonde, Miguel ^{[1
]}

Belen Florez, Ana ^{[1
]}

van Hoek, Angela H. A. M. ^{[2
]}

Stuer-Lauridsen, Birgitte ^{[4
]}

Stroman, Per ^{[3
]}

de los Reyes-Gavilan, Clara G. ^{[1
]}

Margolles, Abelardo ^{[1
]}

机构：

[1] Inst Prod Lacteos Asturias CSIC, Dept Microbiol & Biochem Dairy Prod, Villaviciosa 33300, Asturias, Spain

[2] Wageningen UR, RIKILT Inst Food Safety, Wageningen, Netherlands

[3] Chr Hansen AS, Innovat Cultures & Enzymes Div, Dept Strains, Horsholm, Denmark

[4] Chr Hansen AS, Innovat Cultures & Enzymes Div, Dept Identificat, Horsholm, Denmark

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2010年 / 76卷 / 10期

关键词：

ANTIBIOTIC SUSCEPTIBILITY; PROBIOTIC PRODUCTS; TET(W); BACTERIA; BILE; LACTOBACILLUS; SEQUENCES; STRAINS; HUMANS; THERMOPHILUM;

D O I：

10.1128/AEM.03096-09

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

All strains of Bifidobacterium animalis subsp. lactis described to date show medium level resistance to tetracycline. Screening of 26 strains from a variety of sources revealed the presence of tet(W) in all isolates. A transposase gene upstream of tet(W) was found in all strains, and both genes were cotranscribed in strain IPLAIC4. Mutants with increased tetracycline resistance as well as tetracycline-sensitive mutants of IPLAIC4 were isolated and genetically characterized. The native tet(W) gene was able to restore the resistance phenotype to a mutant with an alteration in tet(W) by functional complementation, indicating that tet(W) is necessary and sufficient for the tetracycline resistance seen in B. animalis subsp. lactis.

引用

页码：3364 / 3369

页数：6

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