Synthesis and characterization of elastin-mimetic protein gels derived from a well-defined polypeptide precursor

被引:118
作者
McMillan, RA [1 ]
Conticello, VP [1 ]
机构
[1] Emory Univ, Dept Chem, Atlanta, GA 30322 USA
关键词
D O I
10.1021/ma9921091
中图分类号
O63 [高分子化学(高聚物)];
学科分类号
070305 ; 080501 ; 081704 ;
摘要
To elucidate the effects of uniformity of molecular architecture on gel properties, a protein polymer based on the elastin-mimetic repeat sequence [(Val-Pro-Gly-Val-Gly)(4)(Val-Pro-Gly-Lys-Gly)], 1 (Lys-25), was synthesized using genetic engineering and microbial protein expression. The regularly placed lysine residues in poly(Lys-25) underwent selective reaction with electrophilic cross-linkers under mild conditions in either dimethyl sulfoxide or aqueous phosphate buffer to afford solvent-swollen networks. Chemical derivatization and spectroscopic investigations of the cross-linking reaction indicated that approximately 85% of the lysine residues reacted with the cross-linker. The protein hydrogel exhibited reversible, temperature-dependent expansion and contraction with an estimated midpoint temperature for the phase transition at 35 degrees C. Scanning electron microscopy (SEM) investigations indicated profound differences in morphology between protein gels prepared in organic vs aqueous solution.
引用
收藏
页码:4809 / 4821
页数:13
相关论文
共 60 条
[1]   Topographic imaging of chromium-coated frozen-hydrated cell and macromolecular complexes by in-lens field emission scanning electron microscopy [J].
Apkarian, RP ;
Caran, KL ;
Robinson, KA .
MICROSCOPY AND MICROANALYSIS, 1999, 5 (03) :197-207
[2]  
Bansil R, 1997, TRENDS POLYM SCI, V5, P146
[3]   Functional domains on elastin and microfibril-associated glycoprotein involved in elastic fibre assembly [J].
BrownAugsburger, P ;
Broekelmann, T ;
Rosenbloom, J ;
Mecham, RP .
BIOCHEMICAL JOURNAL, 1996, 318 :149-155
[4]  
Cappello J., 1994, PLASTICS MICROBES, P35
[5]   NUCLEAR OVERHAUSER EFFECT AND COMPUTATIONAL CHARACTERIZATION OF THE BETA-SPIRAL OF THE POLYPENTAPEPTIDE OF ELASTIN [J].
CHANG, DK ;
VENKATACHALAM, CM ;
PRASAD, KU ;
URRY, DW .
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS, 1989, 6 (05) :851-858
[6]   Colorimetric analysis of surface reactive amino groups on poly(lactic acid-co-lysine):poly(lactic acid) blends [J].
Cook, AD ;
Pajvani, UB ;
Hrkach, JS ;
Cannizzaro, SM ;
Langer, R .
BIOMATERIALS, 1997, 18 (21) :1417-1424
[7]  
Dumitriu Severian, 1994, P3
[8]  
Ferrari F. A., 1997, PROTEIN BASED MAT, P37
[9]  
Flory P J., PRINCIPLES POLYM CHE
[10]  
GEHRKE SH, 1993, ADV POLYM SCI, V110, P81