Genetic typing of classical swine fever virus isolates from the territory of the Czech Republic

被引:28
作者
Bartak, P
Greiser-Wilke, I
机构
[1] State Vet Inst Jihlava, Natl Swine Fever Lab, Jihlava 58605, Czech Republic
[2] Hannover Sch Vet Med, Inst Virol, D-30559 Hannover, Germany
关键词
classical swine fever; epidemiology; wild boar; disease control; genetic typing;
D O I
10.1016/S0378-1135(00)00260-1
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Epizootiological studies based on genetic typing were performed using 14 isolates from outbreaks of classical swine fever (CSF) in domestic pigs and wild boar in the Czech Republic which occurred between 1991 and 1998. They were compared with Austrian, Slovakian, Hungarian, Polish and German isolates. The aim of this study was to characterise the CSF virus isolates and find out the possible relationships between the outbreaks in domestic pigs and wild boar, and to map the spread of the virus in the Czech Republic. For this, fragments of the 5' nontranslated region (5' NTR) and of the E2 glycoprotein gene were sequenced and used for genetic typing. The analysis of both fragments of the genome showed that the Czech isolates belong to two CSF subgroups within group 2, namely to subgroups 2.2 and 2.3. A close relation was found with Austrian isolates from 1992 and 1994, belonging to subgroup 2.2. The isolates in subgroup 2.3 formed a very homogenous group, although they originated from different regions of the country. They seem identical to two Slovakian isolates from 1998, and differed from Hungarian isolates from 1992. Epizootiological links became evident when the epidemiological data were compared. (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:59 / 70
页数:12
相关论文
共 24 条
[1]  
BECHER P, 1997, J GEN VIROL, V78, P1
[2]   PROTEINS ENCODED BY BOVINE VIRAL DIARRHEA VIRUS - THE GENOMIC ORGANIZATION OF A PESTIVIRUS [J].
COLLETT, MS ;
LARSON, R ;
BELZER, SK ;
RETZEL, E .
VIROLOGY, 1988, 165 (01) :200-208
[3]  
Felsenstein J., 1989, CLADISTICS, V5, P164, DOI DOI 10.1111/J.1096-0031.1989.TB00562.X
[4]   Application of a computer program for genetic typing of classical swine fever virus isolates from Germany [J].
Greiser-Wilke, I ;
Depner, K ;
Fritzemeier, J ;
Haas, L ;
Moennig, V .
JOURNAL OF VIROLOGICAL METHODS, 1998, 75 (02) :141-150
[5]   Structure and presentation of a World Wide Web database of CSF virus isolates held at the EU Reference Laboratory [J].
Greiser-Wilke, I ;
Zimmermann, B ;
Fritzemeier, J ;
Floegel, G ;
Moennig, V .
VETERINARY MICROBIOLOGY, 2000, 73 (2-3) :131-136
[6]   RAPID CHARACTERIZATION OF NEW PESTIVIRUS STRAINS BY DIRECT SEQUENCING OF PCR-AMPLIFIED CDNA FROM THE 5' NONCODING REGION [J].
HOFMANN, MA ;
BRECHTBUHL, K ;
STAUBER, N .
ARCHIVES OF VIROLOGY, 1994, 139 (1-2) :217-229
[7]   PRESUMPTIVE DIAGNOSTIC DIFFERENTIATION OF HOG-CHOLERA VIRUS FROM BOVINE VIRAL DIARRHEA AND BORDER DISEASE VIRUSES BY USING A CDNA NESTED-AMPLIFICATION APPROACH [J].
KATZ, JB ;
RIDPATH, JF ;
BOLIN, SR .
JOURNAL OF CLINICAL MICROBIOLOGY, 1993, 31 (03) :565-568
[8]   Classical swine fever virus diversity and evolution [J].
Lowings, P ;
Ibata, G ;
Needham, J ;
Paton, D .
JOURNAL OF GENERAL VIROLOGY, 1996, 77 :1311-1321
[9]   MOLECULAR-CLONING AND NUCLEOTIDE-SEQUENCE OF THE GENOME OF HOG-CHOLERA VIRUS [J].
MEYERS, G ;
RUMENAPF, T ;
THIEL, HJ .
VIROLOGY, 1989, 171 (02) :555-567
[10]  
MEYERS G, 1996, ADV VIRUS RES, V47, P58