Analysis of replication kinetics of the human metapneumovirus in different cell lines by real-time PCR

被引:37
作者
Deffrasnes, C
Côté, S
Boivin, G
机构
[1] CHU Laval, CHUQ, Ste Foy, PQ G1V 4G2, Canada
[2] Univ Laval, Quebec City, PQ G1K 7P4, Canada
[3] CHU Quebec, Res Ctr Infect Dis, Quebec City, PQ G1K 7P4, Canada
关键词
D O I
10.1128/JCM.43.1.488-490.2005
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Human metapneumovirus (hMPV) is associated with acute respiratory tract disease especially in young children. Using a quantitative real-time TaqMan PCR, we analyzed the replication kinetics of hMPV in different cell lines. Our results indicate that hMPV replicates slightly more efficiently in LLC-MK2 than in Vero cells and poorly in HEp-2 cells.
引用
收藏
页码:488 / 490
页数:3
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