Acyl-CoA binding protein is an essential protein in mammalian cell lines

被引：36

作者：

Færgeman, NJ ^{[1
]}

Knudsen, J ^{[1
]}

机构：

[1] Univ So Denmark, Dept Biochem & Mol Biol, DK-5230 Odense M, Denmark

来源：

BIOCHEMICAL JOURNAL | 2002年 / 368卷 / 03期

关键词：

ACBP; acyl-CoA esters; lipid metabolism; small interference RNA (siRNA);

D O I：

10.1042/BJ20021413

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In the present work, small interference RNA was used to knock-down acyl-CoA binding protein (ACBP) in HeLa, HepG2 and Chang cells. Transfection with ACBP-specific siRNA stopped growth, detached cells from the growth surface and blocked thymidine and acetate incorporation. The results show that depletion of ACBP in mammalian cells results in lethality, suggesting that ACBP is an essential protein.

引用

页码：679 / 682

页数：4

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