High antitumor activity using intratumoral injection of plasmid DNA with mutant-type p27Kip1 gene following in vivo electroporation

In this study, we attempted to use a non-viral gene , transfer system, in vivo electroporation. in oral cancer cell B88 xenografts. To evaluate this hi vivo gene transfer method, the GFP gene was transfected into xenografts by electroporation. Then, the efficiency of transfection of C gene by electroporation was confirmed by exogenous p27(Kipl) Western blot analysis. Next, to estimate the reduction of oral cancer xenografts by this method, we measured the size of B88 xenografts in nude mice after electroporation with the wild- or inutant-type p27(Kipl) gene. The growth of tumors was markedly suppressed by mutant-type p27(Kipl) gene transfection by electroporation compared with transfection of wild-type P27(Kipl) gene or empty vector only. Moreover, histological specimens revealed apoptotic cell death was increased in mutant-type p27(Kipl)-transfected tumors compared to wildtype or empty vector only. These results suggest that it is possible to transfer mutant-type p27(Kipl) into oral cancer xenografts using electroporation and to Suppress the growth of tumors, furthermore, it is suggested that this system might be used for oral cancer.