Analysis of cannabinoids by liquid chromatography - Thermospray mass spectrometry and liquid chromatography - Tandem mass spectrometry

被引：10

作者：

Ndjoko, K ^{[1
]}

Wolfender, JL ^{[1
]}

Hostettmann, K ^{[1
]}

机构：

[1] Univ Lausanne, Inst Pharmacognosie & Phytochim, BEP, CH-1015 Lausanne, Switzerland

来源：

CHROMATOGRAPHIA | 1998年 / 47卷 / 1-2期

关键词：

column liquid chromatography mass spectrometry; thermospray; identification and quantification; Delta(9)-tetrahydrocannabinolic acid; Delta(9)-tetrahydrocannabinol; cannabinol; cannabidiol;

D O I：

10.1007/BF02466788

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid method based on liquid chromatography and thermospray mass spectrometry without any derivatization or pre-purification steps has been developed for the identification and quantification of cannabinoids in drugs from cannabis plants. The extracts were separated on a Cls reversed-phase column with an acidic acetonitrile-water gradient. Liquid chromatography-mass spectrometry was performed with a thermospray interface and protonated molecular ions were obtained from the cannabinoids of interest. Liquid chromatography-tandem mass spectrometry experiments on the molecular ions gave additional structural information online. The sensitivity and selectivity of the method was sufficient to enable the detection of 100 pg of the cannabinoids.

引用

页码：72 / 76

页数：5

共 18 条

[1] CONSTITUENTS OF CANNABIS-SATIVA L .12. MASS-SPECTRAL FRAGMENTATION PATTERNS FOR SOME - CANNABINOID ACIDS AS THEIR TMS DERIVATIVES [J].

BILLETS, S ;

ELFERALY, F ;

FETTERMAN, PS ;

TURNER, CE .

ORGANIC MASS SPECTROMETRY, 1976, 11 (07) :741-751

[2]

BJSRKMAN S, 1982, J CHROMATOGR, V237, P389

[3] MASSENSPEKTROSKOPIE UND IHRE ANWENDUNG AUF STRUKTURELLE UND STEREOCHEMISCHE PROBLEME .68. MASSENSPEKTROSKOPISCHE UNTERSUCHUNG DER INHALTSTOFFE VON HASCHISCH [J].

BUDZIKIEWICZ, H ;

ALPIN, RT ;

LIGHTNER, DA ;

DJERASSI, C ;

MECHOULAM, R ;

GAONI, Y .

TETRAHEDRON, 1965, 21 (07) :1881-+

[4] MISSISSIPPI-GROWN CANNABIS-SATIVA L - PRELIMINARY OBSERVATION ON CHEMICAL DEFINITION OF PHENOTYPE AND VARIATIONS IN TETRAHYDROCANNABINOL CONTENT VERSUS AGE, SEX, AND PLANT PART [J].

FETTERMAN, PS ;

KEITH, ES ;

WALLER, CW ;

GUERRERO, O ;

DOORENBOS, NJ ;

QUIMBY, MW .

JOURNAL OF PHARMACEUTICAL SCIENCES, 1971, 60 (08) :1246-+

[5]

FRIEDRICHFIECHTL J, 1975, TETRAHEDRON, V31, P479, DOI 10.1016/0040-4020(75)85016-2

[6] IDENTIFICATION OF MAJOR-DRUGS OF ABUSE USING CHROMATOGRAPHY - AN UPDATE [J].

GOUGH, TA ;

BAKER, PB .

JOURNAL OF CHROMATOGRAPHIC SCIENCE, 1983, 21 (04) :145-153

[7] IDENTIFICATION OF MAJOR-DRUGS OF ABUSE USING CHROMATOGRAPHY [J].

GOUGH, TA ;

BAKER, PB .

JOURNAL OF CHROMATOGRAPHIC SCIENCE, 1982, 20 (07) :289-329

[8]

GOUGH TA, 1991, ANAL DRUGS ABUSE

[9] COMPARISON OF 14 SUBSTITUTED SILYL DERIVATIVES FOR CHARACTERIZATION OF ALCOHOLS, STEROIDS AND CANNABINOIDS BY COMBINED GAS-LIQUID-CHROMATOGRAPHY AND MASS-SPECTROMETRY [J].

HARVEY, DJ .

JOURNAL OF CHROMATOGRAPHY, 1978, 147 (JAN) :291-298

[10]

IRIE H, 1975, CHEM PHARM BULL, V23, P1894

← 1 2 →