Differential gene expression during flowering in the oil palm (Elaeis guineensis)

被引：7

作者：

Singh, R ^{[1
]}

Cheah, SC ^{[1
]}

机构：

[1] Palm Oil Res Inst Malaysia, Kuala Lumpur 50720, Malaysia

来源：

PLANT CELL REPORTS | 2000年 / 19卷 / 08期

关键词：

oil palm; flowering; mRNA fingerprinting;

D O I：

10.1007/s002999900179

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The technique of mRNA fingerprinting was used to isolate newer-specific cDNAs in the oil palm. Differences in the RNA populations between vegetative tissue (leaf) and inflorescences at various stages of flower development were examined using 18 primer combinations. A total of 16 flower-specific cDNAs were identified, of which 15 were successfully re-amplified. Reverse Northern analysis confirmed that 8 of the 15 cDNAs appeared to truly represent differentially expressed mRNAs in flowering tissues. Northern blot analysis subsequently showed that 5 of the clones are preferentially or exclusively expressed in the flowering tissues of oil palm.

引用

页码：804 / 809

页数：6

共 18 条

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