Rapid communication - A novel method for analyzing phosphoproteins using SELDI-TOF MS in combination with a series of recombinant proteins

被引：7

作者：

Akashi, Tetsuyuki ^{[1
]}

Yamori, Takao ^{[1
]}

机构：

[1] Japanese Fdn Canc Res, Ctr Canc Chemotherapy, Div Mol Pharmacol, Koto Ku, Tokyo 1358550, Japan

来源：

PROTEOMICS | 2007年 / 7卷 / 14期

关键词：

phosphoprotein; ribosomal P2; SELDI-TOF MS;

D O I：

10.1002/pmic.200700157

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel SELDI-TOF MS-based method for analyzing phosphoproteins was developed using a series of recombinant wild-type and mutant ribosomal P2 proteins. We demonstrated that the phosphorylation status of the overexpressed proteins in cells was easily and rapidly confirmed using this method. The ribosomal P2 protein contained two phosphorylation sites, which were sequentially phosphorylated in vivo. We also quantitatively detected the phosphoprotein by using SELDI-TOF MS.

引用

页码：2350 / 2354

页数：5

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