M32, a murine homologue of Drosophila heterochromatin protein 1 (HP1), localises to euchromatin within interphase nuclei and is largely excluded from constitutive heterochromatin
CYTOGENETICS AND CELL GENETICS
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1996年
/
73卷
/
04期
关键词:
D O I:
10.1159/000134363
中图分类号:
Q2 [细胞生物学];
学科分类号:
071009 ;
090102 ;
摘要:
Mice possess two structural homologues of Drosophila HP1, termed M31 and M32 (Singh et al., 1991). We have previously shown that an M31-specific monoclonal antibody (MoAb), MAC 353, localises to constitutive heterochromatin (Wreggett et al., 1994). Here we report that a MoAb raised against the M32 protein (MAC 385) recognises a 22-kDa protein in murine nuclear extracts and that M32 is distributed in a fine-grain ''speckled'' pattern within interphase nuclei. M32 is also largely excluded from the large masses of constitutive heterochromatin that are labelled by MAC 353.