M32, a murine homologue of Drosophila heterochromatin protein 1 (HP1), localises to euchromatin within interphase nuclei and is largely excluded from constitutive heterochromatin

被引:78
作者
Horsley, D
Hutchings, A
Butcher, GW
Singh, PB
机构
[1] BABRAHAM INST,DEPT GENET & DEV,CAMBRIDGE CB2 4AT,ENGLAND
[2] BABRAHAM INST,DEPT IMMUNOL,MONOCLONAL ANTIBODY UNIT,CAMBRIDGE CB2 4AT,ENGLAND
来源
CYTOGENETICS AND CELL GENETICS | 1996年 / 73卷 / 04期
关键词
D O I
10.1159/000134363
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Mice possess two structural homologues of Drosophila HP1, termed M31 and M32 (Singh et al., 1991). We have previously shown that an M31-specific monoclonal antibody (MoAb), MAC 353, localises to constitutive heterochromatin (Wreggett et al., 1994). Here we report that a MoAb raised against the M32 protein (MAC 385) recognises a 22-kDa protein in murine nuclear extracts and that M32 is distributed in a fine-grain ''speckled'' pattern within interphase nuclei. M32 is also largely excluded from the large masses of constitutive heterochromatin that are labelled by MAC 353.
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页码:308 / 311
页数:4
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