Characterization of synovial mast cells in knee osteoarthritis: association with clinical parameters

被引：110

作者：

de Lange-Brokaar, B. J. E. ^{[1
]}

Kloppenburg, M. ^{[1
,2
]}

Andersen, S. N. ^{[1
]}

Dorjee, A. L. ^{[1
]}

Yusuf, E. ^{[1
]}

Herb-van Toorn, L. ^{[1
]}

Kroon, H. M. ^{[3
]}

Zuurmond, A. -M. ^{[4
]}

Stojanovic-Susulic, V. ^{[5
]}

Bloem, J. L. ^{[3
]}

Nelissen, R. G. H. H. ^{[6
]}

Toes, R. E. M. ^{[1
]}

Ioan-Facsinay, A. ^{[1
]}

机构：

[1] Leiden Univ, Med Ctr, Dept Rheumatol, C1-38,Postbus 9600, NL-2300 RC Leiden, Netherlands

[2] Leiden Univ, Med Ctr, Dept Clin Epidemiol, NL-2300 RC Leiden, Netherlands

[3] Leiden Univ, Med Ctr, Dept Radiol, NL-2300 RC Leiden, Netherlands

[4] TNO, Leiden, Netherlands

[5] Janssen Res & Dev LLC, Spring House, PA USA

[6] Leiden Univ, Med Ctr, Dept Orthopaed, NL-2300 RC Leiden, Netherlands

来源：

OSTEOARTHRITIS AND CARTILAGE | 2016年 / 24卷 / 04期

关键词：

Osteoarthritis (OA); Mast cells; Inflammation; PROTEASE-ACTIVATED RECEPTOR-2; RHEUMATOID-ARTHRITIS; ANTIRHEUMATIC THERAPY; SYSTEMIC MASTOCYTOSIS; TISSUE INFLAMMATION; CARTILAGE LOSS; EXPRESSION; TRYPTASE; ALPHA; PAIN;

D O I：

10.1016/j.joca.2015.11.011

中图分类号：

R826.8 [整形外科学]; R782.2 [口腔颌面部整形外科学]; R726.2 [小儿整形外科学]; R62 [整形外科学（修复外科学）];

学科分类号：

100224 [整形外科学];

摘要：

Objective: To investigate the presence of mast cells in the osteoarthritic (OA) synovium and their association with clinical parameters in comparison with rheumatoid arthritis (RA) samples. Method: Synovial tissues of 56 symptomatic OA and 49 RA patients were obtained. Two to three paraffin slides were used to quantify inflammation using haematoxylin and eosin (H&E) staining (synovitis score 0-9), and numbers of mast cells (per 10 high-power fields) using double immunofluorescence for CD117 and tryptase. Average scores per patient were used for analysis. Knee radiographs of OA patients were scored according to the Kellgren and Lawrence (KL) system and pain was determined in OA patients at baseline by visual analogue scale (VAS). Results: Median (range) of mast cells was significantly higher in OA samples 45 (1-168) compared to RA samples 4 (1-47) (P-value < 0.001), despite a lower median (range) synovitis score in OA (2.5 (0-6.0)) compared to 4.6 (0-8.0) in RA samples. The synovitis score was significantly correlated with the number of mast cells (in OA Spearman's rho (P-value) 0.3 (0.023) and RA 0.5 (P-value < 0.001)). Interestingly, we observed a trend towards an association between the number of mast cells and an increased KL-grade (P-value 0.05) in OA patients, independently of synovitis. No associations were found with self-reported pain. Conclusion: Prevalence of mast cells in OA synovial tissue is relatively high and associates with structural damage in OA patients, suggesting a role of mast cells in this disease. (C) 2015 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.

引用

页码：664 / 671

页数：8

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