Monocyte chemoattractant protein-1 is generated via TGF-β by myofibroblasts in gastric intestinal metaplasia and carcinoma without H-pylori infection

被引：21

作者：

Mutoh, Hiroyuki ^{[1
]}

Sashikawa, Miho ^{[1
]}

Hayakawa, Hiroko ^{[1
]}

Sugano, Kentaro ^{[1
]}

机构：

[1] Jichi Med Univ, Dept Med, Div Gastroenterol, Shimotsuke, Tochigi, Japan

来源：

CANCER SCIENCE | 2010年 / 101卷 / 08期

关键词：

NECROSIS-FACTOR-ALPHA; COLONIC SUBEPITHELIAL MYOFIBROBLASTS; SMOOTH-MUSCLE-CELLS; MACROPHAGE INFILTRATION; TUMOR VASCULARITY; EPITHELIAL-CELLS; GENE-EXPRESSION; TRANSGENIC MICE; MESSENGER-RNA; MAP-KINASE;

D O I：

10.1111/j.1349-7006.2010.01609.x

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Helicobacter pylori (H. pylori) stimulates secretion of monocyte chemoattractant protein 1 (MCP-1) from gastric mucosa. Monocyte chemoattractant protein-1 (MCP-1) expression and macrophage infiltration are recognized in human gastric carcinoma. We have previously generated Cdx2-transgenic mice as model mice for intestinal metaplasia. Both chronic H. pylori-associated gastritis and Cdx2-transgenic mouse stomach develop intestinal metaplasia and finally gastric carcinoma. In this study we have directed our attention to MCP-1 expression in the intestinal metaplastic mucosa and the gastric carcinoma of Cdx2-transgenic mouse stomach. Quantitative real-time PCR was performed to determine MCP-1 and transforming growth factor-beta 1 (TGF-beta 1) mRNA expression levels and single- or double-label immunohistochemistry was used to evaluate the localization of MCP-1, TGF-beta type I receptor, and alpha-smooth muscle actin (alpha SMA). We determined that MCP-1 mRNA dramatically increased in the intestinal metaplastic mucosa and the gastric carcinoma of Cdx2-transgenic mouse stomach, compared with normal mouse stomach. Both MCP-1 and TGF-beta type I receptor were co-expressed in the alpha SMA-positive myofibroblasts of intestinal metaplastic mucosa and gastric carcinoma. Exogenous application of TGF-beta 1 increased MCP-1 mRNA expression levels in the intestinal metaplastic tissue. Furthermore, TGF-beta 1 was overexpressed and macrophage was strongly infiltrated in the gastric carcinoma. In conclusion, MCP-1 expression, which was stimulated by TGF-beta 1, was recognized in the TGF-beta type I receptor-expressing myofibroblasts of the intestinal metaplastic mucosa and the gastric carcinoma of Cdx2-transgenic mouse stomach. The present results suggest that intestinal metaplasia and gastric carcinoma themselves induce MCP-1 expression independently of H. pylori infection. (Cancer Sci 2010).

引用

页码：1783 / 1789

页数：7

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