We demonstrate simultaneous trapping and spatial separation of the two stable rubidium isotopes in a magneto-optical trap. To realize this novel type of isotope separation, we have built a compact diode laser which is frequency-stabilized by optical feedback from a diffraction grating, and frequency-modulated by a direct microwave modulation of the bias current from a yittrium iron garnet tuned oscillator. Use of the single diode laser with microwave sidebands provides much ease and flexibility in the applications of a magneto-optical trap. (C) 1996 American Institute of Physics.