Reagentless electrochemical immunoassay using electrocatalytic nanoparticle-modified antibodies

被引:19
作者
Polsky, Ronen [1 ]
Harper, Jason C. [1 ]
Wheeler, David R. [1 ]
Dirk, Shawn M. [1 ]
Rawlings, Julia A. [1 ]
Brozik, Susan M. [1 ]
机构
[1] Sandia Natl Labs, Albuquerque, NM 87185 USA
关键词
D O I
10.1039/b704012a
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
We describe a new approach for reagentless electrochemical immunoassay sensing in which Au/Pd NPs can be "loaded'' onto antibodies to create an electrocatalytic antibody that is sensitive to the oxygen reduction reaction.
引用
收藏
页码:2741 / 2743
页数:3
相关论文
共 22 条
[1]   Gold nanoparticle-based quantitative electrochemical detection of amplified human cytomegalovirus DNA using disposable microband electrodes [J].
Authier, L ;
Grossiord, C ;
Brossier, P ;
Limoges, B .
ANALYTICAL CHEMISTRY, 2001, 73 (18) :4450-4456
[2]   Diazonium-protein adducts for graphite electrode microarrays modification: Direct and addressed electrochemical immobilization [J].
Corgier, BP ;
Marquette, CA ;
Blum, LJ .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2005, 127 (51) :18328-18332
[3]   An electrochemical metalloimmunoassay based on a colloidal gold label [J].
Dequaire, M ;
Degrand, C ;
Limoges, B .
ANALYTICAL CHEMISTRY, 2000, 72 (22) :5521-5528
[4]   Electrochemical interrogation of conformational changes as a reagentless method for the sequence-specific detection of DNA [J].
Fan, CH ;
Plaxco, KW ;
Heeger, AJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (16) :9134-9137
[5]   Metal nanoparticles as labels for heterogeneous, chip-based DNA detection [J].
Fritzsche, W ;
Taton, TA .
NANOTECHNOLOGY, 2003, 14 (12) :R63-R73
[6]   Pt nanopartictes functionalized with nucleic acid act as catalytic labels for the chemiluminescent detection of DNA and proteins [J].
Gill, Ron ;
Polsky, Ronen ;
Willner, Itamar .
SMALL, 2006, 2 (8-9) :1037-1041
[7]   Colloidal Au-enhanced surface plasmon resonance for ultrasensitive detection of DNA hybridization [J].
He, L ;
Musick, MD ;
Nicewarner, SR ;
Salinas, FG ;
Benkovic, SJ ;
Natan, MJ ;
Keating, CD .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2000, 122 (38) :9071-9077
[8]   Electroanalytical and bioelectroanalytical systems based on metal and semiconductor nanoparticles [J].
Katz, E ;
Willner, I ;
Wang, J .
ELECTROANALYSIS, 2004, 16 (1-2) :19-44
[9]   Ru-decorated Pt surfaces as model fuel cell electrocatalysts for CO electrooxidation [J].
Maillard, F ;
Lu, GQ ;
Wieckowski, A ;
Stimming, U .
JOURNAL OF PHYSICAL CHEMISTRY B, 2005, 109 (34) :16230-16243
[10]   Oxygen reduction kinetics in low and medium temperature acid environment: Correlation of water activation and surface properties in supported Pt and Pt alloy electrocatalysts [J].
Murthi, VS ;
Urian, RC ;
Mukerjee, S .
JOURNAL OF PHYSICAL CHEMISTRY B, 2004, 108 (30) :11011-11023