Development of a set of near isogenic and backcross recombinant inbred lines containing most of the Lycopersicon hirsutum genome in a L-esculentum genetic background:: A tool for gene mapping and gene discovery

被引:159
作者
Monforte, AJ
Tanksley, SD
机构
[1] Cornell Univ, Dept Plant Breeding, Ithaca, NY 14853 USA
[2] Cornell Univ, Dept Plant Biol, Ithaca, NY 14853 USA
关键词
germplasm; near isogenic line; tomato; genetic resources;
D O I
10.1139/gen-43-5-803
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A novel population consisted of a set of 99 near isogenic lines (NILs) and backcross recombinant inbred lines (BCRILs) derived from a cross between the cultivated tomato Lycopersicon esculentum cv. E6206 and L. hirsutum accession LA1777 is presented. Most of the lines contain a single defined introgression from L. hirsutum in the L. esculentum genetic background and together, the lines provide a coverage of more than the 85% of the L. hirsutum genome. These lines represent a new tool to uncover the genetic resources hidden in L. hirsutum as well as to study the genes responsible of its unique biology. Furthermore, the study of the allelic frequency and heterozygosity among BCRILs showed that specific genomic regions were likely subjected to unintentional selection pressures during the stock development. Genes involved in the reproductive behavior and (or) pollen viability are hypothesized to be responsible for these alterations.
引用
收藏
页码:803 / 813
页数:11
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