The structure of an insect chymotrypsin

被引:53
作者
Botos, I
Meyer, E [1 ]
Nguyen, M
Swanson, SM
Koomen, JM
Russell, DH
Meyer, EF [1 ]
机构
[1] Texas A&M Univ, Dept Biochem & Biophys, College Stn, TX 77843 USA
[2] Texas A&M Univ, Dept Chem, College Stn, TX 77843 USA
关键词
chymotrypsin; fire ant; protein crystallography; serine proteinase; crystal structure;
D O I
10.1006/jmbi.2000.3699
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The South American imported fire ant (Solenopsis invicta), without natural enemies in the United States, widely infests the southern United States, causing more than a half billion dollars in health and agriculture-related damage annually in Texas alone. Fire ants are resistant to most insecticides, so control will require a more fundamental understanding of their biochemistry and metabolism leading to the design of selective, ecologically safe insecticides. The 4th instar larvae play a crucial role in the nutrition of the colony by secreting proteinases (especially chymotrypsin) which digest food products for the entire colony. The first structure of an ant proteolytic enzyme, fire ant chymotrypsin, was determined to atomic resolution (1.7 Angstrom). A structural comparison of the ant and mammalian structures confirms the "universality" of the serine proteinase motif and reveals a difference at residues 147-148, which are proteolytically removed in the bovine enzyme but are firmly intact in the ant chymotrypsin, suggesting a different activation mechanism for the latter. Likewise, the absence of the covalently attached propeptide domain (1-15) further suggests an uncharacteristic activation mechanism. The presence of Gly189 in the S1 site is an atypical feature of this chymotrypsin and is comparable only to human leukocyte elastase, hornet chymo-trypsin and fiddler crab collagenase. Binding studies confirm the chymotrypsin nature of this novel enzyme. (C) 2000 Academic Press.
引用
收藏
页码:895 / 901
页数:7
相关论文
共 38 条
  • [1] Bazan J. F., 1990, SEMIN VIROL, V1, P311
  • [2] STRUCTURE AND MECHANISM OF CHYMOTRYPSIN
    BLOW, DM
    [J]. ACCOUNTS OF CHEMICAL RESEARCH, 1976, 9 (04) : 145 - 152
  • [3] X-RAY CRYSTAL-STRUCTURE OF THE COMPLEX OF HUMAN-LEUKOCYTE ELASTASE (PMN ELASTASE) AND THE 3RD DOMAIN OF THE TURKEY OVOMUCOID INHIBITOR
    BODE, W
    WEI, AZ
    HUBER, R
    MEYER, E
    TRAVIS, J
    NEUMANN, S
    [J]. EMBO JOURNAL, 1986, 5 (10) : 2453 - 2458
  • [4] HUMAN-LEUKOCYTE AND PORCINE PANCREATIC ELASTASE - X-RAY CRYSTAL-STRUCTURES, MECHANISM, SUBSTRATE-SPECIFICITY, AND MECHANISM-BASED INHIBITORS
    BODE, W
    MEYER, E
    POWERS, JC
    [J]. BIOCHEMISTRY, 1989, 28 (05) : 1951 - 1963
  • [5] FREE R-VALUE - A NOVEL STATISTICAL QUANTITY FOR ASSESSING THE ACCURACY OF CRYSTAL-STRUCTURES
    BRUNGER, AT
    [J]. NATURE, 1992, 355 (6359) : 472 - 475
  • [6] Crystallography & NMR system:: A new software suite for macromolecular structure determination
    Brunger, AT
    Adams, PD
    Clore, GM
    DeLano, WL
    Gros, P
    Grosse-Kunstleve, RW
    Jiang, JS
    Kuszewski, J
    Nilges, M
    Pannu, NS
    Read, RJ
    Rice, LM
    Simonson, T
    Warren, GL
    [J]. ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 1998, 54 : 905 - 921
  • [7] THE 2.2-A RESOLUTION X-RAY CRYSTAL-STRUCTURE OF THE COMPLEX OF TRYPSIN INHIBITED BY 4-CHLORO-3-ETHOXY-7-GUANIDINOISOCOUMARIN - A PROPOSED MODEL OF THE THROMBIN INHIBITOR COMPLEX
    CHOW, MM
    MEYER, EF
    BODE, W
    KAM, CM
    RADHAKRISHNAN, R
    VIJAYALAKSHMI, J
    POWERS, JC
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1990, 112 (21) : 7783 - 7789
  • [8] CHRISTOPHER JA, 1998, SPOCK STRUCTURAL PRO
  • [9] ACCURATE BOND AND ANGLE PARAMETERS FOR X-RAY PROTEIN-STRUCTURE REFINEMENT
    ENGH, RA
    HUBER, R
    [J]. ACTA CRYSTALLOGRAPHICA SECTION A, 1991, 47 : 392 - 400
  • [10] COMPARATIVE MODELING METHODS - APPLICATION TO THE FAMILY OF THE MAMMALIAN SERINE PROTEASES
    GREER, J
    [J]. PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS, 1990, 7 (04) : 317 - 334