Sphingosylphosphorylcholine induces Ca2+-sensitization of vascular smooth muscle contraction:: possible involvement of Rho-kinase

被引：50

作者：

Todoroki-Ikeda, N ^{[1
]}

Mizukami, Y ^{[1
]}

Mogami, K ^{[1
]}

Kusuda, T ^{[1
]}

Yamamoto, K ^{[1
]}

Miyake, T ^{[1
]}

Sato, M ^{[1
]}

Suzuki, S ^{[1
]}

Yamagata, H ^{[1
]}

Hokazono, Y ^{[1
]}

Kobayashi, S ^{[1
]}

机构：

[1] Yamaguchi Univ, Sch Med, Dept Physiol 1, Ube, Yamaguchi 7558505, Japan

来源：

FEBS LETTERS | 2000年 / 482卷 / 1-2期

关键词：

sphingolipid; cytosolic Ca2+ concentration; Ca2+ sensitivity; smooth muscle; coronary artery; Y-27632;

D O I：

10.1016/S0014-5793(00)02046-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Sphingosylphosphorylcholine (SPC), a sphingolipid, concentration-dependently (1-50 mu M) induced contraction and slight elevation of the cytosolic Ca2+ concentration ([Ca2+](i)) in smooth muscle of the pig coronary artery, the result being a marked increase in the force/[Ca2+](i) ratio. In alpha-toxin- or beta-escin-permeabilized, but not Triton X-100-permeabilized, vascular strips, SPC induced contraction at constant [Ca2+](i) (pCa 6.3) in the absence of GTP, whereas a G-protein-coupled receptor agonist, histamine, required the presence of GTP to induce the contraction. The Rho-kinase blocker, Y-27632 (10 mu M) abolished the SPC-induced Ca2+-sensitization, without affecting the Ca2+-induced contraction. These results suggest that SPC induces Ca2+-sensitization of force in vascular smooth muscle, presumably through the activation of Rho-kinase (or a related kinase), (C) 2000 Federation of European Biochemical Societies. Published ba Elsevier Science B.V. All rights reserved,

引用

页码：85 / 90

页数：6

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