Ambroxol as an inhibitor of nitric oxide-dependent activation of soluble guanylate cyclase

被引：21

作者：

Severina, IS

Bussygina, OG

Pyatakova, NV

Khropov, YV

Krasnoperov, RA

机构：

[1] Russian Acad Med Sci, Inst Biomed Chem, Moscow 119832, Russia

[2] Moscow State Univ, Sch Biol, Moscow, Russia

[3] Sechenov Moscow Med Acad, Moscow, Russia

来源：

EUROPEAN JOURNAL OF PHARMACOLOGY | 2000年 / 407卷 / 1-2期

基金：

俄罗斯基础研究基金会;

关键词：

guanylate cyclase; nitric oxide (NO); ambroxol;

D O I：

10.1016/S0014-2999(00)00739-1

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The influence of ambroxol on the activity of human platelet soluble guanylate cyclase and rat lung soluble guanylate cyclase was investigated. Ambroxol in the concentration range from 0.1 to 10 muM had no effect on the basal activity of both enzymes and slightly enhanced it at 50 and 100 muM. Ambroxol inhibited in a concentration-dependent manner the sodium nitroprusside-induced activation of both enzymes. The IC50 values for inhibition by ambroxol of sodium nitroprusside-stimulated human platelet soluble guanylate cyclase and rat lung soluble guanylate cyclase were 3.9 and 2.1 muM, respectively. Ambroxol did not influence the stimulation of soluble guanylate cyclase by protoporphyrin 1X. Thus, it is possible that the molecular mechanism of the therapeutic action of ambroxol involves the inhibition of nitric oxide (NO)-dependent activation of soluble guanylate cyclase. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：61 / 64

页数：4

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