Intracellular free calcium concentration and cisplatin resistance in human lung adenocarcinoma A549 cells

被引：39

作者：

Liang, XJ ^{[1
]}

Huang, YG ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China

来源：

BIOSCIENCE REPORTS | 2000年 / 20卷 / 03期

基金：

中国国家自然科学基金;

关键词：

human lung adenocarcinoma A(549) cells; cisplatin resistant; intracellular free Ca2+;

D O I：

10.1023/A:1005530501137

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Human lung adenocarcinoma A(549) cells sensitive and A(549)/DDP cells resistant to Cis-dichlorodiammine platinum[II] (cisplatin) exhibit different intracellular free calcium and calcium fluorescence images labeled with Fura-2/AM and Fluo-3/AM as judged by dual-excitation fluorescence assay, Miracal Imaging and Laser Scanning Confocal Microscopy (LSCM) of single cells. The concentration of intracellular free calcium of the resistant A549/DDP cells is one third that of the sensitive A(549) cells. The efflux of Rhodamine 123 in resistant A(549)/DDP cells is faster than that in sensitive A(549) cells. Tn addition, A(549)/DDP cells have an increase of Phosphatidylinositol 4-kinase (PtdIns 4-kinase) activity in the plasma membrane. So it is tentatively suggested that the increase in PtIns 4-kinase activity resulting from lower intracellular Ca2+ concentration leads to an increase of its enzymatic products-PIP and PIP2, which may stimulate the activity of P-glycoprotein.

引用

页码：129 / 138

页数：10

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