Bioluminometric assay of ATP in mouse brain: Determinant factors for enhanced test sensitivity

被引：34

作者：

Khan, HA ^{[1
]}

机构：

[1] Riyadh Kharj Hosp Program, Dept Med Res, Riyadh 11159, Saudi Arabia

来源：

JOURNAL OF BIOSCIENCES | 2003年 / 28卷 / 04期

关键词：

ATP assay; bioluminescence; mouse brain;

D O I：

10.1007/BF02705114

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Firefly luciferase bioluminescence (FLB) is a highly sensitive and specific method for the analysis of adenosine-5-triphosphate (ATP) in biological samples. Earlier attempts to modify the FLB test for enhanced sensitivity have been typically based on in vitro cell systems. This study reports an optimized FLB procedure for the analysis of ATP in small tissue samples. The results showed that the sensitivity of the FLB test can be enhanced several fold by using ultraturax homogenizer, perchloric acid extraction, neutralization of acid extract and its optimal dilution, before performing the assay reaction.

引用

页码：379 / 382

页数：4

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