Characterization of the presumptive phosphorylation sites of the Bacillus subtilis glucose permease by site-directed mutagenesis:: implication in glucose transport and catabolite repression

被引：10

作者：

Bachem, S ^{[1
]}

Faires, N ^{[1
]}

Stülke, J ^{[1
]}

机构：

[1] Univ Erlangen Nurnberg, Lehrstuhl Mikrobiol, Inst Mikrobiol Biochem & Genet, D-91058 Erlangen, Germany

来源：

FEMS MICROBIOLOGY LETTERS | 1997年 / 156卷 / 02期

关键词：

Bacillus subtilis; protein phosphorylation; glucose uptake; carbon catabolite repression; mutagenesis;

D O I：

10.1111/j.1574-6968.1997.tb12733.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Bacillus subtilis utilizes glucose as the preferred source of carbon and energy. Glucose is transported and concomitantly phosphorylated by the glucose permease (PtsG) of the phosphoenolpyruvate:sugar phosphotransferase system. The phosphate is transferred from enzyme I via HPr and domains IIA and IIB of the glucose permease to the sugar. In this study mutants affected in the putative phosphorylation sites of glucose permease were constructed and the effect on sugar transport and glucose repression tested. Phosphorylation of both domains IIA(Glc) and IIBGlc is required for efficient glucose transport and repression of beta-xylosidase and the bglPH operon.

引用

页码：233 / 238

页数：6

共 27 条

[1] THE BACTERIAL PHOSPHOENOLPYRUVATE-DEPENDENT PHOSPHOTRANSFERASE SYSTEM - ISOLATION OF ACTIVE-SITE PEPTIDES BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AND DETERMINATION OF THEIR PRIMARY STRUCTURE [J].