Isolation and characterization of Brenneria quercina, causal agent for bark canker and drippy nut of Quercus spp. in Spain

被引:48
作者
Biosca, EG
González, R
López-López, MJ
Soria, S
Montón, C
Pérez-Laorga, E
López, MM
机构
[1] Inst Valenciano Invest Agr, Valencia 46113, Spain
[2] Patrimonio Nacl, Madrid 28071, Spain
[3] DARP, Lab Sanitat Vegetal, Barcelona 08040, Spain
[4] Conselleria Medio Ambiente, Serv Gest Forestal, Valencia 46011, Spain
关键词
inoculation; phenotypic; serology;
D O I
10.1094/PHYTO.2003.93.4.485
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The drippy nut disease of oak was first described in California in 1967 and, since then, the causal agent has not been reported in any other area. This study describes for the first time in Europe the isolation of Brenneria (Erwinia) quercina from bark canker in addition to drippy bud and drippy nut in Quercus ilex and Q. pyrenaica. The bark canker and drippy bud symptoms were not previously described as caused by this bacterium. No fungal pathogens were associated with any of the symptoms. Physiological and biochemical characterization identified the pathogenic isolates from Spain as belonging to B. quercina, similar to the reference strain CFBP 1266. Fatty acid profiles of the Spanish isolates also were similar to the strain of B. quercina from California. Serological analysis by indirect immunofluorescence and enzyme-linked immunosorbent assay using polyclonal antisera against the reference strain of B. quercina and one Spanish oak isolate revealed some antigenic heterogeneity between isolates of different origins. Pathogenicity tests demonstrated that the Spanish isolates were able to reproduce internal symptoms of necrosis and acorn exudation in Q. ilex and Q. pyrenaica and suggest that B. quercina may be associated, among other causes, with the oak decline syndrome affecting Spanish oak forests.
引用
收藏
页码:485 / 492
页数:8
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