Proliferation and apoptosis of fibroblasts and smooth muscle cells in rat uterine cervix throughout gestation and the effect of the antiprogesterone onapristone

被引:40
作者
Leppert, P
机构
[1] SUNY Buffalo, Dept Obstet Gynecol, Buffalo, NY USA
[2] Univ Rochester, Rochester Gen Hosp, Dept Obstet & Gynecol, Rochester, NY 14627 USA
关键词
cervix; apoptosis; proliferation; antiprogesterone; collagen; rat;
D O I
10.1016/S0002-9378(98)70481-8
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
OBJECTIVE: The purpose of this study was to determine the relative percentages of apoptosis and proliferation in fibroblasts and cervical smooth muscles throughout gestation and the effect of an antiprogesterone on these processes. STUDY DESIGN: Rats were studied at days 5, 15, 18, and 21 and immediately postpartum (day 22). Apoptosis and proliferation as detected by specific immunohistochemistry quantitative morphometric analysis was performed. Onapristone, an antiprogesterone, was used to study effects of hormonal change on these processes in 16- and 19-day timed-pregnant rats. RESULTS: Proliferation of fibroblasts and smooth muscle cells was highest in early pregnancy and decreased progressively, whereas apoptosis increased progressively in later pregnancy. Onapristone inhibited apoptosis. CONCLUSION: Changes in cervical cellular turnover are initiated early in gestation and are under hormonal influence. Antiprogesterone inhibits cell death at days 16 and 19 of gestation.
引用
收藏
页码:713 / 725
页数:13
相关论文
共 42 条
[1]  
BARKAY CG, 1993, AM J OBSTET GYNECOL, V169, P625
[2]  
CHWALISZ K, 1991, RES PER MED, V10, P119
[3]  
COHEN JJ, 1984, J IMMUNOL, V132, P38
[4]  
CRAMER SF, 1995, MODERN PATHOL, V8, P71
[5]   Cell suicide in health and disease [J].
Duke, RC ;
Ojcius, DM ;
Young, JDE .
SCIENTIFIC AMERICAN, 1996, 275 (06) :80-87
[6]   IDENTIFICATION OF PROGRAMMED CELL-DEATH INSITU VIA SPECIFIC LABELING OF NUCLEAR-DNA FRAGMENTATION [J].
GAVRIELI, Y ;
SHERMAN, Y ;
BENSASSON, SA .
JOURNAL OF CELL BIOLOGY, 1992, 119 (03) :493-501
[7]   Preprogrammed and programmed cell death mechanisms of apoptosis: UV-induced immediate and delayed apoptosis [J].
Godar, DE .
PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1996, 63 (06) :825-830
[8]   AN ENHANCEMENT METHOD FOR IMMUNOHISTOCHEMICAL STAINING OF PROLIFERATING CELL NUCLEAR ANTIGEN IN ARCHIVAL RODENT TISSUES [J].
GREENWELL, A ;
FOLEY, JF ;
MARONPOT, RR .
CANCER LETTERS, 1991, 59 (03) :251-256
[9]   APOPTOSIS IN DECIDUAL TISSUE REGRESSION AND REORGANIZATION [J].
GU, Y ;
JOW, GM ;
MOULTON, BC ;
LEE, C ;
SENSIBAR, JA ;
PARKSARGE, OK ;
CHEN, TJ ;
GIBORI, G .
ENDOCRINOLOGY, 1994, 135 (03) :1272-1279
[10]   RIPENING OF THE UTERINE CERVIX OF THE GUINEA-PIG AFTER TREATMENT WITH THE PROGESTERONE ANTAGONIST ONAPRISTONE (ZK-98.299) - AN ELECTRON-MICROSCOPIC STUDY [J].
HEGELEHARTUNG, C ;
CHWALISZ, K ;
BEIER, HM ;
ELGER, W .
HUMAN REPRODUCTION, 1989, 4 (04) :369-377